MULTIPLE FUNCTIONS OF SEGMENT POLARITY GENES IN DROSOPHILA
MULTIPLE FUNCTIONS OF SEGMENT POLARITY GENES IN DROSOPHILA
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DOI：
10.1016/0012-1606(87)90061-3
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发表时间：
1987-02-01
影响因子：
2.7
通讯作者：
MAHOWALD, AP
中科院分区：
文献类型：
--
作者：
PERRIMON, N;MAHOWALD, AP
1（1）dsh是一种晚期合子致死突变，表现出可挽救的母体效应致死表型。来自具有纯合l（1）dsh生殖系克隆的雌性的l（1）dsh/Y胚胎表现出节段极性胚胎表型。对这些胚胎发育的分析表明：（1）尽管形成了正确数量的气管小凹，但节段边界没有形成;（2）在气管小凹之间发生细胞死亡的口袋;（3）在胚带缩短期间，爬行表达变得异常。我们认为，在缺乏母体和合子的表达l（1）dsh+，细胞从每个后室死亡。随后，来自前室的细胞必须重新排列它们的位置值以产生节段极性表型。我们比较了l（1）dsh的表型与其他五个体节极性基因座的表型：四个胚胎致死基因[l（1）犰狳，l（2）醋栗，l（2）无翅，和l（3）刺猬];和晚合子致死基因l（1）融合。只有l（2）无翅胚表现出与来自纯合生殖系克隆的l（1）dsh/Y胚相似的早期分节缺陷。相反，在l（1）犰狳、l（2）醋栗、l（3）刺猬和l（1）融合胚中，分割基本正常。各自的母亲和合子的贡献和作用的片段极性基因座的图案的胚胎和成人进行了讨论。
l(1)dishevelled (l(1)dsh) is a late zygotic lethal mutation that exhibits a rescuable maternal effect lethal phenotype. l(1)dsh/Y embryos, derived from females possessing a homozygous l(1)dsh germline clone, exhibit a segment polarity embryonic phenotype. Analysis of the development of these embryos indicates: (1) that segmental boundaries do not form although the correct number of tracheal pits is formed; (2) that pockets of cell death occur between the tracheal pits; and (3) that engrailed expression becomes abnormal during germ band shortening. We propose that, in the absence of both maternal and zygotic expression of l(1)dsh+, cells from each posterior compartment die. Subsequently, cells from the anterior compartment must rearrange their positional values to generate the segment polarity phenotype. We have compared the phenotype of l(1)dsh with the phenotype of five other segment polarity loci: four embryonic lethals [l(1)armadillo, l(2)gooseberry, l(2)wingless, and l(3)hedgehog]; and the late zygotic lethal, l(1)fused. Only l(2)wingless embryos exhibit early segmentation defects similar to those found in l(1)dsh/Y embryos derived from homozygous germline clones. In contrast, segmentation is esentially normal in l(1)armadillo, l(2)gooseberry, l(3)hedgehog, and l(1)fused embryos. The respective maternal and zygotic contribution and the roles of the segment polarity loci for the patterning of the embryo and the adult are discussed.