A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia.
A New Imaging Platform for Visualizing Biological Effects of Non-Invasive Radiofrequency Electric-Field Cancer Hyperthermia.
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DOI：
10.1371/journal.pone.0136382
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Serda RE
中科院分区：
文献类型：
--
作者：
Corr SJ;Shamsudeen S;Vergara LA;Ho JC;Ware MJ;Keshishian V;Yokoi K;Savage DJ;Meraz IM;Kaluarachchi W;Cisneros BT;Raoof M;Nguyen DT;Zhang Y;Wilson LJ;Summers H;Rees P;Curley SA;Serda RE
在此，我们提出了一种新的成像平台来研究无创射频（RF）电场癌症热疗的生物效应。该系统允许实时在体内活体显微镜（IVM）成像的射频诱导的生物学改变，如血管结构和药物灌注的变化。我们的研究结果表明，IVM系统能够处理暴露于高功率电场，而不会引起显着的硬件损坏或成像伪影。此外，在低于41°C的温度下，短时间的低功率（< 200 W）射频暴露增加了荧光示踪剂进入肿瘤的运输和灌注。肿瘤温度约为44°C时，可见血管变形和血液凝固。这些结果强调了我们的集成IVM-RF成像平台作为一种强大的新工具的使用，可以可视化射频能量与生物组织，器官和肿瘤之间的动态和相互作用。
Herein, we present a novel imaging platform to study the biological effects of non-invasive radiofrequency (RF) electric field cancer hyperthermia. This system allows for real-time in vivo intravital microscopy (IVM) imaging of radiofrequency-induced biological alterations such as changes in vessel structure and drug perfusion. Our results indicate that the IVM system is able to handle exposure to high-power electric-fields without inducing significant hardware damage or imaging artifacts. Furthermore, short durations of low-power (< 200 W) radiofrequency exposure increased transport and perfusion of fluorescent tracers into the tumors at temperatures below 41°C. Vessel deformations and blood coagulation were seen for tumor temperatures around 44°C. These results highlight the use of our integrated IVM-RF imaging platform as a powerful new tool to visualize the dynamics and interplay between radiofrequency energy and biological tissues, organs, and tumors.