Scirrhous changes in dysplastic nodules do not indicate high-grade status
Scirrhous changes in dysplastic nodules do not indicate high-grade status
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DOI：
10.1046/j.1440-1746.2003.03052.x
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发表时间：
2003-06-01
影响因子：
4.1
通讯作者：
Theise, N
中科院分区：
文献类型：
--
作者：
An, HJ;Illei, P;Theise, N
背景和目的：发育不良结节（DN）可分为高级别和低级别，前者被称为癌前或交界性病变。最近报道了有关这些类型 DN 的许多形态特征。在本研究中，我们基于使用 Ki-67 免疫组织化学染色进行的形态学和细胞动力学分析，尝试评估 DN 的硬性改变作为高级别 DN 的指示性特征。方法：我们回顾了 35 例 DN 肝脏，并选择了 15 例伴有硬性改变的 DN。我们用苏木精和伊红、三色、网状蛋白和 Perls 染色剂对每个病例的 DN 承载切片进行染色。我们尝试根据纤维化模式对硬质改变进行细分和表征。我们还用Ki-67免疫组化染色来评估具有硬变的DN的增殖活性。结果：我们发现了两种类型的硬变，即细胞周型和星状型。细胞周围型与形成马洛里体的胆汁淤积变性有关，而星状型与可能由缺血性损伤引起的广泛门静脉纤维化有关。在伴有硬变的 DN 中，高级别 DN 包含 5 个结节（33%），低级别结节 10 个（67%）。硬性改变的存在或类型与 DN 分级之间没有显着关系。本研究未显示硬变类型之间 Ki-67 标记指数的显着差异。我们也无法发现硬质 DN（高级别和低级别）的 Ki-67 标记指数与周围再生结节的 Ki-67 标记指数之间的差异。结论：该证据表明 DN 的硬质变化并不是高级别 DN 的特定特征。我们还发现硬质DN有两种可能代表生物学不同过程的形态变异，即细胞周硬质型和星状硬质型。 (C) 2003 年布莱克威尔出版亚洲有限公司。
Background and Aims: Dysplastic nodules (DN) may be divided into high-grade and low-grade, and the former has been known as a precancerous or borderline lesion. Recently many morphological characteristics concerning these types of DN have been reported. In the present study we attempted to evaluate the scirrhous change in DN as an indicative feature of high-grade DN, based on the morphological and cell-kinetic analyses using immunohistochemical stains for Ki-67.Methods: We reviewed 35 livers with DN and selected 15 DN with scirrhous change. We stained DN-bearing sections of each case with hematoxylin and eosin, trichrome, reticulin and Perls' stain. We tried to subclassify and characterize the scirrhous change according to the fibrosis pattern. We also stained with Ki-67 immunohistochemically to assess the proliferative activity of DN with scirrhous change.Results: We found two types of scirrhous change, that is, pericellular and stellate. The pericellular type was related to the Mallory body-forming cholestatic degeneration, whereas the stellate type was associated with extensive portal fibrosis probably induced by ischemic damage. Among DN with scirrhous change, high-grade DN comprised five nodules (33%) and there were 10 (67%) low-grade nodules. There was no significant relationship between the presence or the types of scirrhous change and the grade of DN. The significant differences of Ki-67 labeling indices between types of scirrhous change were not shown in this study. We also could not find the differences between Ki-67 labeling indices of scirrhous DN (high and low grades) and those of surrounding regenerative nodules.Conclusions: This evidence indicated that the scirrhous change in DN was not a specific feature of high-grade DN. We also found that scirrhous DN have two morphological varieties that may represent biologically different processes, that is, pericellular scirrhous type and stellate scirrhous type. (C) 2003 Blackwell Publishing Asia Pty Ltd.