Neurological assessment scores in rabbit embolic stroke models.
Neurological assessment scores in rabbit embolic stroke models.
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DOI：
10.2174/1874205x01307010038
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Culp, William C
中科院分区：
文献类型：
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作者：
Brown, Aliza;Woods, Sean;Culp, William C
背景：神经结局和行为评估在卒中动物模型中被广泛应用，但在兔模型中的评估并未得到充分验证。方法：麻醉新西兰大白兔(N=131只，4~5 kg)经颈内动脉插管置入颈内动脉栓塞术，观察动脉闭塞情况。在栓塞后24小时用0(正常)到10(死亡)的NAS测试对兔进行评估。缺陷标准包括颈部扭转、翻正反射、后爪和前爪伸展反射和姿势。用三苯基四氮唑(TTC)染色的脑切片分析%IV。测量并计算脑梗塞体积占总脑体积的百分比。
BACKGROUND: Neurological outcomes and behavioral assessments are widely used in animal models of stroke, but assessments in rabbit models are not fully validated. The wryneck model of neurological assessment scores (NAS) was compared to percent infarct volume (%IV) values (infarct volume is a proven clinical indicator of stroke severity) and arterial occlusion localization in three rabbit angiographic stroke models.HYPOTHESIS: NAS values will correlate with percent infarct volume values.METHODS: Anesthetized New Zealand White rabbits (N=131, 4-5 kg) received internal carotid artery emboli by angiographic catheter introduced into the femoral artery and occlusions were characterized. Rabbits were evaluated at 24 hours post embolism using the NAS test of 0 (normal) to 10 (death). Deficit criteria included neck twist, righting reflex, extension reflex in hind paw and forepaw, and posture. Brain sections stained with triphenyltetrazolium chloride (TTC) were analyzed for %IV. Volume of the infarct was measured and calculated as a percent of the total brain volume.RESULTS: The aggregate correlation for NAS values vs. %IV values was R=0.61, p