Effects of fasting and refeeding on ob gene expression in white adipose tissue of lean and obese (ob/ob) mice
Effects of fasting and refeeding on ob gene expression in white adipose tissue of lean and obese (ob/ob) mice
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DOI：
10.1016/0014-5793(95)00719-p
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发表时间：
1995-07
期刊：
影响因子：
3.5
通讯作者：
P. Trayhurn;Moira E. A. Thomas;J. Duncan;Vernon Rayner
中科院分区：
文献类型：
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作者：
P. Trayhurn;Moira E. A. Thomas;J. Duncan;Vernon Rayner
一个33聚体的反义寡核苷酸已被用作探针的快速荧光为基础的检测ob（肥胖）mRNA。ob基因的表达在小鼠的几个白色脂肪组织库中是明显的（附睾，最高;皮下和网膜，最低），但在其他器官中没有。禁食（24小时）诱导瘦小鼠附睾脂肪中ob mRNA的大幅下降，这在再喂养时迅速逆转，与ob在能量平衡中的假定作用一致。然而，禁食对肥胖（ob/ob）小鼠的ob mRNA水平没有影响。
A 33-mer antisense oligonucleotide has been utilized as a probe for the rapid chemiluminescence-based detection of ob (obese) mRNA. Expression of the ob gene was evident in several white adipose tissue depots of mice (epididymal, highest; subcutaneous and omental, lowest), but not in other organs. Fasting (24 h) induced a substantial fall in ob mRNA in the epididymal fat of lean mice, which was rapidly reversed on refeeding, responses consistent with the putative role of ob in energy balance. Fasting had no effect, however, on ob mRNA levels in obese (ob/ob) mice.