Supercritical fluid chromatography with fused-silica packed columns
Supercritical fluid chromatography with fused-silica packed columns
复制标题
采用熔融石英填充柱的超临界流体色谱
DOI：
10.1016/s0021-9673(01)87633-0
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
F. Nakata
中科院分区：
文献类型：
--
作者：
Y. Hirata;F. Nakata
采用超临界流体色谱法，以200 μm的I.D.熔融石英填充柱和初步结果。使用小口径柱优于大口径柱的优点是低流速，这允许直接加热分析柱中的移动的相。因此，简化了色谱系统的仪器和操作。以正己烷-乙醇为移动的流动相，采用硅胶柱，对聚苯乙烯低聚物进行了分离。还描述了改性剂对留着率的影响。
Supercritical fluid chromatography has been carried out with a 200-μm I.D. fused silica packed column and initial results are presented. The advantage of using a small bore column over a wide bore column is the low flow-rate which allows direct heating of the mobile phase in the analytical column. Consequently, the instrumentation and operation of the chromatographic system are simplified. The separation ability of the system has been demonstrated for polystyrene oligomers using a silica gel column andn-hexane—ethanol as the mobile phase. The effect of modifiers on retention is also described.