Localization of chloride secretion in rabbit colon: inhibition by anthracene-9-carboxylic acid.
Localization of chloride secretion in rabbit colon: inhibition by anthracene-9-carboxylic acid.
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兔结肠中氯化物分泌的定位：蒽-9-羧酸的抑制。
DOI：
10.1152/ajpgi.1986.250.2.g185
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Duffey,ME
中科院分区：
文献类型：
--
作者：
Horvath,PJ;Ferriola,PC;Weiser,MM;Duffey,ME
用取代芳香族化合物蒽-9-羧酸（A-9-C）抑制短路家兔远端结肠上皮细胞的主动Cl-分泌。将组织固定在Ussing室中，并通过向血清浴中加入1 mM二丁酰腺苷3 '，5'-环一磷酸来刺激分泌Cl-。36 Cl-通量测量的结果表明，添加0.1 mM A-9-C的粘膜浴抑制Cl-分泌的48%。通过使用常规微电极确定Cl-分泌的位点，以表明隐窝区域的细胞而不是表面上皮细胞通过顶膜电阻分数从0.75增加到0.80以及顶膜的超极化从-64 mV增加到-68 mV（P小于0.05）来响应A-9-C。将巯基试剂二硫苏糖醇添加到粘膜组织浴中以去除这些组织的隐窝区域的杯状细胞产生的粘液。通过去除粘液屏障，A-9-C对Cl-分泌的最大抑制时间从30分钟缩短至15分钟。A-9-C对隐窝细胞的作用，以及粘液对该化合物抑制作用的作用，证明隐窝区域是Cl-分泌的位点。
The substituted aromatic compound anthracene-9-carboxylic acid (A-9-C) was used to inhibit active Cl- secretion by the epithelium of short-circuited rabbit distal colon. Tissues were mounted in Ussing chambers and stimulated to secrete Cl- by the addition of 1 mM dibutyryl adenosine 3',5'-cyclic monophosphate to the serosal bath. Results of 36Cl-flux measurements showed that the addition of 0.1 mM A-9-C to the mucosal bath inhibited Cl- secretion by 48%. The site of Cl- secretion was determined by using conventional micro-electrodes to show that the cells of the crypt regions, and not the surface epithelial cells, responded to A-9-C by an increase in apical membrane fractional resistance from 0.75 to 0.80 and a hyperpolarization of the apical membrane from -64 to -68 mV (P less than 0.05). The sulfhydryl reagent dithiothreitol was added to the mucosal tissue bath to remove the mucus produced by goblet cells of the crypt regions of these tissues. The time for maximal inhibition of Cl- secretion by A-9-C was decreased from 30 to 15 min by removal of the mucus barrier. The effects of A-9-C on the crypt cells, as well as the effect of mucus on the inhibitory action of this compound, demonstrate that the crypt region is the site of Cl- secretion.