Decreased Immunoreactivity of Hepatitis E Virus Antigen Following Treatment with Sakhalin Spruce (Picea glehnii) Essential Oil
Decreased Immunoreactivity of Hepatitis E Virus Antigen Following Treatment with Sakhalin Spruce (Picea glehnii) Essential Oil
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DOI：
10.1002/cbdv.202200924
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发表时间：
2023-03
影响因子：
2.9
通讯作者：
N. Maeda;Shouya Horochi;Y. Hasegawa;T. Iwasaki;N. Nakatani;T. Miyasho;K. Hagiwara;H. Yokota;Y. Funatsu
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文献类型：
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作者：
N. Maeda;Shouya Horochi;Y. Hasegawa;T. Iwasaki;N. Nakatani;T. Miyasho;K. Hagiwara;H. Yokota;Y. Funatsu
戊型肝炎病毒（HEV）是一种常见的传染病，感染猪、野猪、鹿和人类。在大多数情况下，人类通过食用生肉感染。据报道，一些精油具有抗病毒活性。在这项研究中，为了研究精油的抗HEV特性，在用各种精油处理HEV抗原后，分析了HEV抗原蛋白对相关抗体的免疫反应性。从茶树中提取的精油，以前曾报道具有抗病毒活性，熏衣草和柠檬的活性大大降低。我们发现，用从库页岛云杉制备的精油处理与测试物质中HEV抗原蛋白免疫反应性的最强降低相关。结果表明，库页岛云杉精油的主要挥发性成分为乙酸正丁酯（32.30%）、α-蒎烯（16.66%）、异辛烯（11.14%）、樟脑（5.52%）、β-水芹烯（9.09%）、异辛烯（4.77%）和柠檬烯（4.57%）。研究了精油的各种成分的抗HEV特性：用库页岛云杉油的主要成分乙酸正丁酯、茶树油的主要成分α-蒎烯和柠檬油的主要成分柠檬烯处理，导致HEV抗原免疫反应性大幅降低。这些结果表明，精油的每种主要成分在降低HEV抗原蛋白的免疫反应性方面都起着重要作用;它们还表明库页岛云杉精油具有抗HEV活性。在具有消除食源性感染中HEV感染性的潜力的配方中，这种精油可用作肉类加工和烹饪用具（如刀和砧板）的灭活剂。
The hepatitis E virus (HEV) causes a common infectious disease that infects pigs, wild boars, deer, and humans. In most cases, humans are infected by eating raw meat. Some essential oils have been reported to exhibit antiviral activities. In this study, in order to investigate the anti‐HEV properties of essential oils, the immunoreactivities of HEV antigen proteins against the relevant antibodies were analyzed after the HEV antigens underwent treatment with various essential oils. The essential oils extracted from the tea tree, which was previously reported to exhibit antiviral activity, lavender, and lemon had strongly reduced activity. We found that treatment with the essential oil prepared from Sakhalin spruce was associated with the strongest reduction in immunoreactivity of HEV antigen protein(s) among the tested substances. The main volatile constituents of Sakhalin spruce essential oil were found to be bornyl acetate (32.30 %), α‐pinene (16.66 %), camphene (11.14 %), camphor (5.52 %), β‐phellandrene (9.09 %), borneol (4.77 %), and limonene (4.57 %). The anti‐HEV properties of the various components of the essential oils were examined: treatment with bornyl acetate, the main component of Sakhalin spruce oil, α‐pinene, the main component of tea tree oil, and limonene, the main component of lemon oil, resulted in a strong reduction in HEV antigen immunoreactivity. These results indicate that each main component of the essential oils plays an important role in the reduction of the immunoreactivity of HEV antigen protein(s); they also suggest that Sakhalin spruce essential oil exhibits anti‐HEV activity. In a formulation with the potential to eliminate the infectivity of HEV in foodborne infections, this essential oil can be applied as an inactivating agent for meat processing and cooking utensils, such as knives and chopping boards.