Investigation of the therapeutic mechanism of subthreshold micropulse laser irradiation in retina
Investigation of the therapeutic mechanism of subthreshold micropulse laser irradiation in retina
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DOI：
10.1007/s00417-020-04638-3
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发表时间：
2020-05-01
影响因子：
2.7
通讯作者：
Murata, Toshinori
中科院分区：
文献类型：
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作者：
Hirabayashi, Kazutaka;Kakihara, Shinji;Murata, Toshinori
目的阈下微脉冲激光照射已用于治疗视网膜水肿；但对其治疗作用的机制却鲜有报道。在这项研究中，我们比较了阈值短脉冲和亚阈值微脉冲激光照射小鼠并研究了它们的机制。方法本研究使用9至12周龄的雄性C57BL/6J小鼠。全身麻醉后，使用IQ577对右眼进行阈值短脉冲或亚阈值微脉冲激光照射。激光照射24小时后进行去核，并进行组织学和基因表达分析。结果阈值短脉冲激光照射后可观察到视网膜色素上皮的凝固斑和萎缩，而阈下微脉冲激光照射后未观察到视网膜色素上皮的凝固斑和萎缩。激光治疗 24 小时后，与未治疗对照组相比，阈值短脉冲激光组的水通道蛋白 (AQP) 1、2、7 和 11 水平显着升高 1.7 至 3 倍。 AQP 3 显着增加 100 倍。 VEGF-A 和 VEGFR2 分别上调 1.5 倍和 2.3 倍。在阈下微脉冲激光组中，AQP 3 与未治疗对照组相比增加了6倍。血管生成素-1 和肾上腺髓质素 (AM) 受体 CLR 分别降低 0.6 倍和 0.5 倍。结论阈值短脉冲激光照射引起视网膜损伤，多种基因表达发生显着变化。相反，阈下微脉冲激光照射不会引起视网膜损伤；它上调了 AQP 3，这可能通过视网膜下液的引流改善了视网膜水肿。
Purpose Subthreshold micropulse laser irradiation has been used for the treatment of retinal edema; however, there are few reports about the mechanism of its therapeutic effect. In this study, we compared threshold short pulse and subthreshold micropulse laser irradiation in mice and investigated their mechanism. Methods Nine to 12-week-old male C57BL/6J mice were used in this study. After general anesthesia, threshold short pulse or subthreshold micropulse laser irradiation was performed on the right eye using IQ577. Enucleation was performed 24 h after the laser irradiation, and histological and gene expression analyses were carried out. Results Coagulation spots and atrophy of the retinal pigment epithelium were observed after threshold short pulse laser irradiation but not after subthreshold micropulse laser irradiation. Twenty-four hours after laser, aquaporin (AQP) 1, 2, 7, and 11 levels were significantly elevated by 1.7- to 3-fold in the threshold short pulse laser group compared with non-treated control group. AQP 3 was increased significantly and prominently by 100-fold. VEGF-A and VEGFR2 were upregulated 1.5- and 2.3-fold, respectively. In the subthreshold micropulse laser group, AQP 3 was increased by 6-fold compared with the non-treated control group. Angiopoietin-1 and the adrenomedullin (AM) receptor CLR were decreased by 0.6-fold and 0.5-fold, respectively. Conclusion Threshold short pulse laser irradiation caused retinal damage and prominent changes in the expression of various genes. Contrarily, subthreshold micropulse laser irradiation did not induce retinal damage; it upregulated AQP 3, which might have improved retinal edema by drainage of subretinal fluid.