Surface activity of rabbit pulmonary surfactant subfractions at different concentrations in a captive bubble.
Surface activity of rabbit pulmonary surfactant subfractions at different concentrations in a captive bubble.
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圈养气泡中不同浓度的兔肺表面活性剂亚组分的表面活性。
DOI：
10.1152/jappl.1994.77.2.597
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Clements,JA
中科院分区：
文献类型：
--
作者：
Putz,G;Goerke,J;Clements,JA
本研究在一种新的压力驱动捕获气泡表面计中研究了兔肺表面活性剂亚组分在不同浓度下的表面活性，该表面计提供了比迄今为止应用于该材料的更严格的测试条件。通过离心将洗出的亚组分分离成第三个（P3； 1000 x平均g， 20分钟），第四个（P4； 60000 x平均g， 60分钟）和第五个（P5； 100000 x平均g， 16小时）颗粒。在2.0 mg磷脂/ml时，P3和P4表现出比P5更快的吸附速度，第一次和后续压缩时的最小表面张力更低，单层可压缩性更低。在磷脂浓度为0.1 ~ 2.0 mg /ml之间，P3和P4的表面活性增加。吸附速率常数大约比解吸常数快10000倍。我们得出结论，在正常肺中，P3和P4的成分决定肺泡表面张力。我们推测，在特殊情况下，即使表面活性较低的P5也会对空气-水界面产生重要影响。
This study investigates the surface activity of rabbit pulmonary surfactant subfractions at different concentrations in a new pressure-driven captive bubble surfactometer, which provides more rigorous testing conditions than heretofore applied to this material. Subfractions were separated by centrifugation of lavage return into a third (P3; 1,000 x average g, 20 min), a fourth (P4; 60,000 x average g, 60 min), and a fifth (P5; 100,000 x average g, 16 h) pellet. At 2.0 mg phospholipid/ml, P3 and P4 demonstrated more rapid adsorption, lower minimum surface tensions on first and subsequent compressions, and lower monolayer compressibilities than did P5. This surface activity of P3 and P4 increased with concentration between 0.1 and 2.0 mg phospholipid/ml. Adsorption rate constants were approximately 10,000 times faster than desorption constants. We conclude that, in a normal lung, components of P3 and P4 determine alveolar surface tension. We speculate that under special circumstances even the less surface active P5 could have an important influence at the air-water interface.