Comparative Transcriptome Analyses Revealed Conserved and Novel Responses to Cold and Freezing Stress in Brassica napus L
Comparative Transcriptome Analyses Revealed Conserved and Novel Responses to Cold and Freezing Stress in Brassica napus L
复制标题
比较转录组分析揭示了甘蓝型油菜对寒冷和冷冻胁迫的保守和新颖的反应
DOI：
10.1534/g3.119.400229
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发表时间：
2019-08-01
影响因子：
2.6
通讯作者：
Wei, Hua
中科院分区：
文献类型：
--
作者：
Xin, He;Ni Xianchao;Wei, Hua
油菜籽（Brassica napus L.）是典型的冬季二年生植物，在营养阶段具有较高的耐寒性。近年来，早熟油菜籽品种在全国范围内的种植越来越多。而耐冷性较低的早熟油菜品种在寒冷的冬春季节遭受冻害的风险较高。油菜籽应对不同低温胁迫条件的分子机制尚不清楚。在低温（4°）和低温（- 4°）条件下，对两个不同耐寒性的早熟油菜籽品种的47,328个差异表达基因（DEGs）进行了研究，并对冷驯化和对照条件下的低温和冰冻胁迫进行了研究。京都基因与基因组百科全书（KEGG）分析表明，两条保守的（初级代谢和植物激素信号转导）和两条新的（植物-病原体相互作用途径和昼夜节律途径）信号通路显著富集了差异表达的转录本。研究结果为了解油菜籽低温胁迫响应机制奠定了基础。提出了油菜抗寒性遗传改良的新思路和候选基因。
Oil rapeseed (Brassica napus L.) is a typical winter biennial plant, with high cold tolerance during vegetative stage. In recent years, more and more early-maturing rapeseed varieties were planted across China. Unfortunately, the early-maturing rapeseed varieties with low cold tolerance have higher risk of freeze injury in cold winter and spring. Little is known about the molecular mechanisms for coping with different low-temperature stress conditions in rapeseed. In this study, we investigated 47,328 differentially expressed genes (DEGs) of two early-maturing rapeseed varieties with different cold tolerance treated with cold shock at chilling (4°) and freezing (−4°) temperatures, as well as chilling and freezing stress following cold acclimation or control conditions. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that two conserved (the primary metabolism and plant hormone signal transduction) and two novel (plant-pathogen interaction pathway and circadian rhythms pathway) signaling pathways were significantly enriched with differentially-expressed transcripts. Our results provided a foundation for understanding the low-temperature stress response mechanisms of rapeseed. We also propose new ideas and candidate genes for genetic improvement of rapeseed tolerance to cold stresses.