In Vivo Dopamine Measurements in the Nucleus Accumbens After Nonanesthetic and Anesthetic Doses of Propofol in Rats
In Vivo Dopamine Measurements in the Nucleus Accumbens After Nonanesthetic and Anesthetic Doses of Propofol in Rats
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DOI：
10.1213/00000539-200210000-00022
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发表时间：
2002-10
影响因子：
5.7
通讯作者：
L. Pain;S. Gobaille;Carmen Schleef;D. Aunis;P. Oberling
中科院分区：
文献类型：
--
作者：
L. Pain;S. Gobaille;Carmen Schleef;D. Aunis;P. Oberling
越来越多的证据表明，异丙酚对情感和奖励过程起作用。我们设计这项研究是为了评估异丙酚对中边缘系统的主要组成部分伏隔核多巴胺浓度的影响。采用活体脑微透析法测定自由活动大鼠伏隔核内多巴胺浓度。微透析探针被放置在先前在立体定向手术中放置的引导管中。通过探针灌注液体，每隔20 min采集一次样品，用高压液相色谱法测定浓度。所有大鼠作为自己的对照，根据内部设计随机分为四组，分别腹腔注射不同剂量的异丙酚：0、9、60或100 mg/kg。与基线值相比，多巴胺浓度在最小剂量为9 mg/kg时下降，而在亚麻醉（60 mg/kg）和麻醉（100 mg/kg）剂量时浓度大幅增加。亚麻醉剂量和麻醉剂量的增加幅度相同（+90%），但麻醉剂量的增加时间更长。数据显示，只有亚麻醉和麻醉剂量的异丙酚才会增加伏隔核中多巴胺的浓度，正如之前所描述的潜在滥用药物。
There is growing evidence that propofol acts on affective and reward processes. We designed this study to assess the effect of propofol on the concentration of dopamine in the nucleus accumbens, a main component of the mesolimbic system. The concentration of dopamine in the nucleus accumbens was assessed by using in vivo brain microdialysis in freely moving rats. A microdialysis probe was placed within guide cannulae previously placed during stereotaxic surgery. Fluid was perfused through the probe, and samples were collected every 20 min for measuring concentrations by high-pressure liquid chromatography. All rats served as their own controls and were randomized to four different doses of propofol, injected intraperitoneally: 0, 9, 60, or 100 mg/kg, according to a within design. Compared with the baseline value, dopamine concentration was decreased at the smallest dose of 9 mg/kg, whereas concentration was largely increased at the subanesthetic (60 mg/kg) and anesthetic (100 mg/kg) doses. This increase was of the same magnitude (+90%) for subanesthetic and anesthetic doses but was more prolonged at the anesthetic dose. Data show that only subanesthetic and anesthetic doses of propofol increase the concentration of dopamine in the nucleus accumbens, as previously described with drugs of potential abuse.