Changes in placental dipeptidyl peptidase IV in preeclampsia with intrauterine growth restriction
Changes in placental dipeptidyl peptidase IV in preeclampsia with intrauterine growth restriction
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DOI：
10.1055/s-2005-870229
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发表时间：
2005-07-01
影响因子：
2.2
通讯作者：
Kikkawa, F
中科院分区：
文献类型：
--
作者：
Nishikawa, M;Itakura, A;Kikkawa, F
本研究的目的是检查胎盘表达的二肽基肽酶IV（DPPIV）的变化。DPPIV的定位进行了比较，在控制和先兆子痫胎盘。还测量酶活性、mRNA和蛋白质表达。在足月胎盘中，DPPIV优先在干绒毛内的胎儿血管内皮细胞中表达，而在绒毛间质细胞中仅弱表达。对照胎盘中的DPPIV活性在整个妊娠期间没有显着变化。在正常血压对照病例和患有先兆子痫伴OF但无宫内生长受限的妇女的样品中，活性水平分别为11.8 +/-2.1、13.4 +/- 1.1和15.3 +/- 0.62 pmol pNA/min/mg蛋白。因此，具有宫内生长受限的先兆子痫胎盘显示出比对照组显著更高的活性水平（p < 0.05）。我们认为胎盘DPPIV通过降解胎儿胎盘循环生物活性肽（包括肠促胰岛素）影响胎儿代谢，从而调节胎儿生长。
The purpose of this study was to examine alterations in placental expression of dipeptidyl peptidase IV (DPPIV). The localization of DPPIV was compared in control and preeclamptic placentas. Enzyme activity, mRNA, and protein expression were also measured. in term placentas, DPPIV was expressed preferentially in the fetal vascular endothelial cells within stem villi and only weakly in the villous stromal cells. DPPIV activity in control placentas showed no remarkable changes throughout gestation. Levels of activity in samples from normotensive control cases and women having preeclampsia with OF without intrauterine growth restriction were 11.8 +/- 2.1,13.4 +/- 1.1, and 15.3 +/- 0.62 pmol pNA/min/mg protein, respectively. The preeclamptic placentas with intrauterine growth restriction thus showed significantly higher levels of activity than the controls (p < 0.05). We propose that placental DPPIV influences fetal metabolism via the degradation of fetoplacental circulating bioactive peptides, including incretins, resulting in the regulation of fetal growth.