A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells
A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells
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DOI：
10.1073/pnas.93.5.1820
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发表时间：
1996-03-05
影响因子：
11.1
通讯作者：
Buus, S
中科院分区：
文献类型：
--
作者：
Andersen, PS;Stryhn, A;Buus, S
T 细胞受体对肽/主要组织相容性复合物 (MHC) 分子复合物的特异性识别是特异性免疫反应中的关键反应。因此，针对肽/MHC 复合物的抗体将成为研究 MHC 功能和 T 细胞识别的宝贵工具，并可能带来免疫治疗的新方法。然而，事实证明，通过传统的杂交瘤技术很难产生具有T细胞特异性的抗体。在这里，我们报告说，噬菌体展示技术是生成识别特定的、预定的肽/MHC 复合物的抗体的可行替代方案。
Specific recognition of peptide/major histocompatibility complex (MHC) molecule complexes by the T-cell receptor is a key reaction in the specific immune response. Antibodies against peptide/MHC complexes would therefore be valuable tools in studying MHC function and T-cell recognition and might lead to novel approaches in immunotherapy. However, it has proven difficult to generate antibodies with the specificity of T cells by conventional hybridoma techniques. Here we report that the phage display technology is a Feasible alternative to generate antibodies recognizing specific, predetermined peptide/MHC complexes.