Guidance of optic axons in vivo by a preformed adhesive pathway on neuroepithelial endfeet.
Guidance of optic axons in vivo by a preformed adhesive pathway on neuroepithelial endfeet.
复制标题
通过神经上皮末足上预先形成的粘附通路对体内视轴突进行引导。
DOI：
10.1016/0012-1606(84)90248-3
复制
发表时间：
1984
影响因子：
2.7
通讯作者：
Rutishauser,U
中科院分区：
文献类型：
--
作者：
Silver,J;Rutishauser,U
抗神经细胞粘附分子（NCAM）的抗体在体内用于定位鸡视觉系统发育组织中的NCAM抗原决定簇，并在视神经生长至顶盖期间干扰细胞-细胞粘附。免疫组化研究显示，神经上皮细胞的染色模式，这符合某些地区的视神经轴突的推定路线，不仅相对于从眼睛到顶盖的整体路径，而且在优先分布的边缘endfeet的抗原接触视神经轴突生长锥。抗体干扰研究，其中涉及眼内注射抗NCAM Fab在胚胎的第3.5天，表明抑制NCAM介导的粘附的结果在生长锥神经上皮细胞的关系，因此在视觉通路的显着扭曲。总之，这些研究表明，视神经轴突沿着脑边缘的引导至少部分地受到与NCAM抗原相关的神经上皮细胞上预先形成的粘附通路的影响。
Antibodies against the neural cell adhesion molecule (NCAM) were usedin vivoboth to localize NCAM antigenic determinants in developing tissues of the chicken visual system and to perturb cell-cell adhesion during growth of optic fibers to the tectum. The immunohistochemical studies revealed a staining pattern on neuroepithelial cells which coincided with certain regions of the presumptive route for optic axons, not only with respect to the overall pathway from the eye to the tectum, but also in the preferential distribution of the antigen on the marginal endfeet which are contacted by optic axon growth cones. The antibody-perturbation studies, which involved intraocular injection of anti-NCAM Fab at embryonic Day 3.5, demonstrated that inhibition of NCAM-mediated adhesion results in a dramatic distortion of growth cone-neuroepithelial cell relationships and consequently of the optic pathway. Together, these studies suggest that guidance of optic axons along the margin of the brain is at least in part influenced by a preformed adhesive pathway on neuroepithelial cells associated with NCAM antigens.