Dynamics of Apolipoprotein B-100 in Interaction with Detergent Probed by Incoherent Neutron Scattering
Dynamics of Apolipoprotein B-100 in Interaction with Detergent Probed by Incoherent Neutron Scattering
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DOI：
10.1021/acs.jpclett.1c03141
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发表时间：
2021-12-23
影响因子：
5.7
通讯作者：
Peters, Judith
中科院分区：
文献类型：
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作者：
Cisse, Aline;Schachner-Nedherer, Anna-Laurence;Peters, Judith
载脂蛋白B-100(apo B-100)是低密度脂蛋白和极低密度脂蛋白的蛋白质部分，对胆固醇和甘油三酯的运输起着至关重要的作用。针对目前研究较少的载脂蛋白B-100分子动力学细节，我们结合不同的仪器和时间尺度进行了弹性和准弹性非相干中子散射(EINS，QENS)实验。与经典的膜蛋白类似，增溶作用会产生剩余的洗涤剂，这里是诺尼特P-40(NP40)。因此，我们提出了一个蛋白质-洗涤剂复合体的QENS研究框架，引入了一个组合模型，包括实验的apo B-100/NP40比率。依赖于对所有QENS振幅的同时分析，这种方法足够灵敏，可以将这两种贡献分开。它的应用确定了两点：(1)载脂蛋白B-100的慢动力学；(2)在载脂蛋白B-100存在的情况下NP40动力学的加速。直接翻译暴露的方法学现在使用中子光谱学研究更多的膜蛋白成为可能。
Apolipoprotein B-100 (apo B-100) is the protein moiety of both low- and very-low-density lipoproteins, whose role is crucial to cholesterol and triglyceride transport. Aiming at the molecular dynamics' details of apo B-100, scarcely studied, we performed elastic and quasi-elastic incoherent neutron scattering (EINS, QENS) experiments combining different instruments and time scales. Similar to classical membrane proteins, the solubilization results in remaining detergent, here Nonidet P-40 (NP40). Therefore, we propose a framework for QENS studies of protein-detergent complexes, with the introduction of a combined model, including the experimental apo B-100/NP40 ratio. Relying on the simultaneous analysis of all QENS amplitudes, this approach is sensitive enough to separate both contributions. Its application identified two points: (i) apo B-100 slow dynamics and (ii) the acceleration of NP40 dynamics in the presence of apo B-100. Direct translation of the exposed methodology now makes the investigation of more membrane proteins by neutron spectroscopy achievable.