Immunoglobulin heavy chain germ‐line JH−Cμ transcription in human precursor B lymphocytes initiates in a unique region upstream of DQ52
Immunoglobulin heavy chain germ‐line JH−Cμ transcription in human precursor B lymphocytes initiates in a unique region upstream of DQ52
复制标题
人前体 B 淋巴细胞中免疫球蛋白重链种系 JH−Cμ 转录起始于 DQ52 上游的独特区域
DOI：
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发表时间：
1995
影响因子：
5.4
通讯作者：
R. Hendriks
中科院分区：
文献类型：
--
作者：
A. Thompson;E. Timmers;R. Schuurman;R. Hendriks
从在生殖系构型中具有两个免疫球蛋白（IG）重（H）链基因座的人前体B细胞中，分离各种IgH链生殖系转录物并测序。这些转录本显示含有源自JH区、IgH链增强子元件或IG开关区的序列。许多分离的cDNA克隆在其5′端含有来自DQ 52上游的单个外显子的序列，命名为μ°元件。对含有μ°外显子及其5′侧翼区的920 bp基因组DNA片段的序列分析显示，存在DNA结合蛋白的各种保守基序，如E2 A、Ets、NF-χB和AP-2，这些基序先前已在IgH和L链增强子中发现。我们提出，导致DQ 52-JH基因片段的生殖系转录的μ°元件的活性是产生V（D）J重组酶的完全可及性所必需的。
From human precursor B cells which had both immunoglobulin (Ig) heavy (H) chain loci in germ‐line configuration, various IgH chain germ‐line transcripts were isolated and sequenced. These transcripts were shown to contain sequences derived from the JH region, the IgH chain enhancer element or the Ig switch region. A number of isolated cDNA clones contained sequences at their 5′ end that were derived from a single exon located just upstream of DQ52, designated the μ° element. Sequence analysis of a 920‐bp genomic DNA segment, containing the μ° exon and its 5′ flanking region, revealed the presence of various conserved motifs for DNA‐binding proteins, such as E2A, Ets, NF‐χB and AP‐2, which have previously been found in the IgH and L chain enhancers. We propose that the activity of μ° element, resulting in germ‐line transcription of the DQ52‐JH gene segment, is required to generate full accessibility for the V(D)J recombinase.
影响因子：
56.9
作者：
OETTINGER, MA;SCHATZ, DG;BALTIMORE, D
通讯作者：
BALTIMORE, D
影响因子：
10.5
作者：
SCHLISSEL, M;VORONOVA, A;BALTIMORE, D
通讯作者：
BALTIMORE, D