Periaxin expression in myelinating Schwann cells: modulation by axon-glial interactions and polarized localization during development.
Periaxin expression in myelinating Schwann cells: modulation by axon-glial interactions and polarized localization during development.
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发表时间：
1995-12
期刊：
影响因子：
4.6
通讯作者：
Steven S. Scherer;Yi-Tian Xu;Peter Bannerman;D. Sherman;Peter J. Brophy
中科院分区：
文献类型：
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作者：
Steven S. Scherer;Yi-Tian Xu;Peter Bannerman;D. Sherman;Peter J. Brophy
Periaxin 是一种新描述的蛋白质，仅由髓鞘雪旺细胞表达。在发育中的神经中，周蛋白首先在雪旺细胞包裹轴突时被检测到，然后出现代表髓鞘的蛋白质。 Periaxin 最初集中在轴突膜（与轴突并列）中，但在发育过程中，随着髓鞘的成熟，Periaxin 变得主要集中在轴突雪旺细胞膜（与基底层并列）。在永久性轴突切断的成年神经中，periaxin从轴突和轴突膜上丢失，与退化的髓鞘相关联并被巨噬细胞吞噬。在轴突再生并重新髓鞘化的压碎神经中，当雪旺细胞包裹再生轴突时，周蛋白首先在轴突膜中被检测到，但同样是在其他髓磷脂蛋白出现之前。在永久性轴索切断和挤压后，Periaxin mRNA 和蛋白质水平与其他髓磷脂相关基因的变化平行。这些数据表明，periaxin 由髓鞘雪旺细胞以动态的、发育调节的方式表达。髓鞘形成螺旋化阶段完成后，periaxin 在雪旺细胞中定位的转变表明，periaxin 参与稳定成熟髓鞘所需的膜蛋白相互作用。
Periaxin is a newly described protein that is expressed exclusively by myelinating Schwann cells. In developing nerves, periaxin is first detected as Schwann cells ensheathe axons, prior to the appearance of the proteins that characterize the myelin sheath. Periaxin is initially concentrated in the adaxonal membrane (apposing the axon) but, during development, as myelin sheaths mature, periaxin becomes predominately localized at the abaxonal Schwann cell membrane (apposing the basal lamina). In permanently axotomized adult nerves, periaxin is lost from the abaxonal and adaxonal membranes, becomes associated with degenerating myelin sheaths and is phagocytosed by macrophages. In crushed nerves, in which axons regenerate and are remyelinated, periaxin is first detected in the adoxonal membrane as Schwann cells ensheathe regenerating axons, but again prior to the appearance of other myelin proteins. Periaxin mRNA and protein levels change in parallel with those of other myelin-related genes after permanent axotomy and crush. These data demonstrate that periaxin is expressed by myelinating Schwann cells in a dynamic, developmentally regulated manner. The shift in localization of periaxin in the Schwann cell after completion of the spiralization phase of myelination suggests that periaxin participates in membrane-protein interactions that are required to stabilize the mature myelin sheath.