Dihydropyridine receptor isoform expression in adult rat skeletal muscle.
Dihydropyridine receptor isoform expression in adult rat skeletal muscle.
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成年大鼠骨骼肌中二氢吡啶受体亚型的表达。
DOI：
10.1007/s004240050637
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Palade,P
中科院分区：
文献类型：
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作者：
Péréon,Y;Dettbarn,C;Lu,Y;Westlund,KN;Zhang,JT;Palade,P
使用 RNase 保护测定法研究了大鼠膈肌、比目鱼肌和趾长伸肌中亚型特异性二氢吡啶受体 Ca2+通道 (DHPR) α1 亚基基因的表达。正如预期的那样，DHPR 骨骼肌亚型的 mRNA 表达水平在趾长伸肌中最高。出乎意料的是，膈肌和比目鱼肌均以显着水平表达心脏亚型的 mRNA。此外，免疫组织化学实验提供了肌纤维中蛋白质水平上的心脏 DHPR 亚型的证据。比目鱼肌和膈肌中心脏 DHPR 的存在与这些肌肉中报道的类似心脏的兴奋-收缩耦合的程度一致，并且可能是茶碱对哮喘患者的一些治疗作用的解释，但也可能发挥一些其他作用。
The expression of isoform-specific dihydropyridine receptor Ca2+channel (DHPR) α1-subunit genes in rat diaphragm, soleus and extensor digitorum longus muscles was investigated using RNase protection assays. As expected, mRNA expression levels for the DHPR skeletal muscle isoform were highest in extensor digitorum longus. Unexpectedly, both diaphragm and soleus expressed mRNA for the cardiac isoform at a significant level. Moreover, immunohistochemical experiments provided evidence of the cardiac DHPR isoform at the protein level in muscle fibres. The presence of the cardiac DHPR in the soleus and diaphragm is consistent with a degree of reported cardiac-like excitation-contraction coupling in these muscles, and may be an explanation for some of the therapeutic effects of theophylline in asthmatics, but is likely to serve some other role(s) as well.