Identification and characterization of anthocyanins by high-performance liquid chromatography-electrospray ionization-mass spectrometry in herbaceous peony species
Identification and characterization of anthocyanins by high-performance liquid chromatography-electrospray ionization-mass spectrometry in herbaceous peony species
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高效液相色谱-电喷雾电离-质谱法鉴定芍药中花色苷的含量
DOI：
10.21273/jashs.133.3.418
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发表时间：
2008-05-01
影响因子：
1.9
通讯作者：
Tilt, Kenneth Michael
中科院分区：
文献类型：
--
作者：
Jia, Ni;Shu, Qing-Yan;Tilt, Kenneth Michael
采用高效液相色谱-电喷雾质谱联用技术（HPLC-MS）和二极管阵列检测器（DAD）对9种野生芍药（Escherichia L.）种（15份）。根据HPLC保留时间、洗脱顺序、MS碎片模式，并通过与真实标准和公开数据的比较来鉴定单个花青素。结果表明，该植物中检测到6种主要花色苷：芍药苷-3，5-二-O-葡萄糖苷、芍药苷-3-O-葡萄糖苷-5-O-阿拉伯糖苷（Pn 3G 5Ara）、芍药苷-3-O-葡萄糖苷、天竺葵苷-3，5-二-O-葡萄糖苷、矢车菊苷-3，5-二-O-葡萄糖苷和矢车菊苷-3-O-葡萄糖苷（Cy 3G）。除已知的主要花色苷外，还首次从芍药中鉴定出少量花色苷。两种植物中均含有花青素-3-O-葡萄糖苷-5-O-半乳糖苷和天竺葵素-3-O-葡萄糖苷-5-O-半乳糖苷。和P. anomala ssp.维奇（林奇）Hong & K.Y. Pn 3G 5Ara仅在欧洲野生种和亚种中发现，表明欧洲和中国种质之间存在不同的代谢途径。首次在紫菀属植物中发现了半乳糖和阿拉伯糖结合的花色素苷。北美种，细叶番荔枝，花瓣中Cy 3G含量较高。这种花色素苷成分与亚洲和欧洲物种中的花色素苷成分不同，可能是花中鲜艳的红色的原因。
Petal anthocyanins were systematically identified and characterized by high-performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry (MS) coupled with diode array detection among nine wild herbaceous peony (Paeonia L.) species (15 accessions). Individual anthocyanins were identified according to the HPLC retention time, elution order, MS fragmentation patterns, and by comparison with authentic standards and published data. Six main anthocyanins, including peonidin-3,5-di-O-glucoside, peonidin-3-O-glucoside-5-O-arabinoside (Pn3G5Ara), peonidin-3-O-glucoside, pelargonidin-3,5-di-O-glucoside, cyanidin-3,5-di-O-glucoside, and cyanidin-3-O-glucoside (Cy3G), were detected. In addition to the well-known major anthocyanins, some minor anthocyanins were identified in herbaceous peony species for the first time. Detection of the unique anthocyanins cyanidin-3-O-glucoside-5-O-galactoside and pelargonidin-3-O-glucoside-5-O-galactoside in both Paeonia anomala L. and P. anomala ssp. veitchii (Lynch) D.Y. Hong & K.Y. Pan indicated these two species should belong to the same taxon. Pn3G5Ara was found only in European wild species and subspecies suggesting different metabolic pathways between European and Chinese accessions. Anthocyanins conjugated with galactose and arabinose were observed in the genus Paeonia for the first time. The North American species, Paeonia tenuifolia L., had high Cy3G content in flower petals. This anthocyanin composition is distinct from the anthocyanin composition in Asian and European species and possibly is responsible for the vivid red coloration in flowers.