Real-time monitoring of strand-displacement DNA amplification by a contactless electrochemical microsystem using interdigitated electrodes
Real-time monitoring of strand-displacement DNA amplification by a contactless electrochemical microsystem using interdigitated electrodes
复制标题
DOI：
10.1039/c2lc40384f
复制
发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Zhao, Jianlong
中科院分区：
文献类型：
--
作者：
Fang, Xinxin;Zhang, Huanqian;Zhao, Jianlong
本文报道了一种非接触式电导检测系统的设计和实现，该系统结合了热控制池，数据处理系统和电化学（EC）池的无标记恒温核酸扩增和实时监测。EC电池由微室和交叉指型电极组成，作为非接触式电导率生物传感器，盖玻片作为绝缘体。在我们的工作中，非接触式EC测量，海藻糖对扩增的影响，和芯片表面处理进行了研究。由于生物传感器的上级性能，该装置可以检测浓度小于0.1 pg μ l（-1）的纯DNA的量。的EC细胞，集成了加热器和温度传感器，已成功地实现了切口为基础的链置换扩增在初始浓度为2.5 μ M和产量直接监测（解雇使用探针或标签）在线。这种非接触式探测器具有重要的优点：抗干扰能力强，探测器寿命长，可重复使用性高，成本低。此外，检测单元的小尺寸、低功耗和便携性使该系统具有高度集成的潜力，可用于现场服务和护理点应用。
This paper reports the design and implementation of a contactless conductivity detection system which combines a thermal control cell, a data processing system and an electrochemical (EC) cell for label-free isothermal nucleic acid amplification and real-time monitoring. The EC cell consists of a microchamber and interdigitated electrodes as the contactless conductivity biosensor with a cover slip as insulation. In our work, contactless EC measurements, the effects of trehalose on amplification, and chip surface treatment are investigated. With the superior performance of the biosensor, the device can detect the amount of pure DNA at concentrations less than 0.1 pg mu l(-1). The EC cell, integrated with a heater and a temperature sensor, has successfully implemented nicking-based strand-displacement amplification at an initial concentration of 2.5 mu M and the yields are monitored directly (dismissing the use of probes or labels) on-line. This contactless detector carries important advantages: high anti-interference capability, long detector life, high reusability and low cost. In addition, the small size, low power consumption and portability of the detection cell give the system the potential to be highly integrated for use in field service and point of care applications.