Generation of a Polyclonal Antibody against the Mouse Metal Transporter ZIP8.
Generation of a Polyclonal Antibody against the Mouse Metal Transporter ZIP8.
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DOI：
10.3390/antib10020016
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发表时间：
2021-04-21
期刊：
影响因子：
--
通讯作者：
Zhao N
中科院分区：
文献类型：
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作者：
Wei G;Wu Y;Zhao N
ZIP8是一种新发现的金属转运蛋白。在人类患者中，ZIP8的突变导致严重的锰缺乏，这表明ZIP8在调节全身锰稳态中起着关键作用。在小鼠中，ZIP8的缺失重现了ZIP8突变患者的症状。然而，由于缺乏合适的抗体检测内源性ZIP8蛋白，阻碍了利用小鼠模型研究ZIP8功能的进一步研究。在这项研究中，我们报告了针对小鼠ZIP8的多克隆抗体的设计，生成和验证。我们已经证明，新产生的抗体可以可靠地用于免疫印迹分析，以检测小鼠组织中的内源性ZIP8蛋白。抗小鼠ZIP8抗体的成功产生和验证为进一步研究这种金属转运蛋白的功能和调控提供了机会。此外，我们的研究可能为靶向多位膜蛋白的抗体的未来发展提供有价值的见解。
ZIP8 is a newly identified metal transporter. In human patients, mutations in ZIP8 result in severe manganese deficiency, suggesting a critical role for ZIP8 in regulating systemic manganese homeostasis. In mice, the deletion of ZIP8 recapitulates the symptoms of patients with ZIP8 mutations. However, further studies using mouse models to examine ZIP8′s function were hindered by the lack of suitable antibodies to detect endogenous ZIP8 protein. In this study, we report the design, generation, and validation of a polyclonal antibody against mouse ZIP8. We have demonstrated that the newly generated antibody can be reliably used in immunoblotting analysis to detect endogenous ZIP8 protein in mouse tissues. The successful generation and validation of anti-mouse ZIP8 antibody provide opportunities to further examine the function and regulation of this metal transporter. In addition, our study may provide valuable insights into the future development of antibodies targeting polytopic membrane proteins.