Expression of ferritin protein and subunit mRNAs in normal and iron deficient rat brain
Expression of ferritin protein and subunit mRNAs in normal and iron deficient rat brain
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DOI：
10.1016/s0169-328x(99)00011-x
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发表时间：
1999-03-05
期刊：
影响因子：
--
通讯作者：
Moos, T
中科院分区：
文献类型：
--
作者：
Hansen, TM;Nielsen, H;Moos, T
在非神经组织中，铁蛋白亚基mRNA受转录后机制调节，导致铁缺乏期间铁蛋白合成减少。生物化学研究表明，在缺铁期间，脑铁蛋白浓度下降，表明脑中铁蛋白亚基mRNA的表达可能受到与非神经组织相似的机制的调节。然而，由于铁蛋白在大脑中的表达仅得到模糊的研究，因此这一假设仍有待验证。我们研究了饮食铁缺乏对铁蛋白的细胞分布的影响，采用免疫组织化学和H-和L-铁蛋白亚基mRNA的非放射性原位杂交。从受孕之日起，对妊娠大鼠进行铁耗竭饮食（6.4 mg/kg）。窝仔保持相同的饮食，直到在出生后10周龄时实施安乐死。这种治疗使脑铁水平从约57 μ g/g降低到26 μ g/g。减少铁储备减少了组织化学可检测的铁和铁蛋白免疫反应性的表达在神经元，少突胶质细胞样和小胶质细胞样细胞。在正常大鼠中，H-和L-铁蛋白亚基mRNA几乎在所有神经元和非神经元细胞中表达。铁蛋白亚基mRNA的脑表达不受铁缺乏的影响。当通过北方印迹法检测时，脑中铁蛋白亚基mRNA的水平也未因缺铁而改变。总之，铁和铁蛋白质的脑水平是非常容易受到膳食缺铁，而大脑表达的H-和L-铁蛋白亚基mRNA保持不变。(C)1999 Elsevier Science B. V.保留所有权利。
In non-neuronal tissue, ferritin subunit mRNAs are regulated by post-transcriptional mechanisms leading to decreased ferritin protein synthesis during iron deficiency. Biochemical studies have demonstrated that the cerebral ferritin concentration declines during iron deficiency, suggesting that expression of ferritin subunit mRNAs in the brain may be regulated by mechanisms similar to those of non-neuronal tissue. However, as ferritin expression has been only vaguely studied in brain, this hypothesis remains to be tested. We investigated the influence of dietary iron deficiency on the cellular distribution of ferritin protein using immunohistochemistry and H- and L-ferritin subunit mRNAs by non-radioactive in situ hybridization. Pregnant rats were subjected to an iron depleted diet (6.4 mg/kg) from the day of conception. Litters were kept on the same diet until euthanized at the postnatal age of 10 weeks. This treatment reduced brain iron levels from approximately 57 to 26 mu g/g. Reducing the iron stores reduced histochemical detectable iron and the expression of ferritin immunoreactivity in neurons, oligodendrocyte-like and microglia-like cells. In normal rats, H- and L-ferritin subunit mRNAs were expressed in virtually all neurons and non-neuronal cells. The cerebral expression of the ferritin subunit mRNAs was not affected by iron deficiency. The levels of ferritin subunit mRNAs in the brain were also unaltered from iron deficiency when examined by Northern blotting. In conclusion, brain levels of iron and ferritin protein are highly susceptible to dietary iron deficiency, whereas the cerebral expression of H- and L-ferritin subunit mRNAs remains unchanged. (C) 1999 Elsevier Science B.V. All rights reserved.