Structures of KOD and 9°N DNA Polymerases Complexed with Primer Template Duplex
Structures of KOD and 9°N DNA Polymerases Complexed with Primer Template Duplex
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DOI：
10.1002/cbic.201300175
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发表时间：
2013-06-17
期刊：
影响因子：
3.2
通讯作者：
Marx, Andreas
中科院分区：
文献类型：
--
作者：
Bergen, Konrad;Betz, Karin;Marx, Andreas
KOD和9n DNA聚合酶是两种广泛用于复制高修饰核苷酸DNA的酶，它们的结构在引物/模板双工复合物中得到了高分辨率的解析。这些数据阐明了两种酶的底物相互作用，并为进一步优化酶和底物铺平了道路。
Structures of KOD and 9 N DNA polymerases, two enzymes that are widely used to replicate DNA with highly modified nucleotides, were solved at high resolution in complex with primer/template duplex. The data elucidate substrate interaction of the two enzymes and pave the way for further optimisation of the enzymes and substrates.