Ultrastable and Biocompatible NIR-II Quantum Dots for Functional Bioimaging
Ultrastable and Biocompatible NIR-II Quantum Dots for Functional Bioimaging
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用于功能生物成像的超稳定且生物相容性 NIR-II 量子点
DOI：
10.1002/adfm.201703451
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发表时间：
2018
影响因子：
19
通讯作者：
Qian Jun
中科院分区：
文献类型：
--
作者：
Zebibula Abudureheman;Alifu Nuernisha;Xia Liqun;Sun Chaowei;Yu Xiaoming;Xue Dingwei;Liu Liwei;Li Gonghui;Qian Jun
近红外第二光谱区（NIR - II, 1000-1700 nm）的荧光生物成像由于光散射小，具有空间分辨率高、穿透深度大的优点。然而，同时具有高亮度、良好稳定性和生物相容性的NIR - II荧光团是非常罕见的。疏水性NIR - II发射PbS@CdS量子点（QDs）通过二氧化硅和两亲性聚合物（Pluronic F - 127）双层涂层方法被表面功能化。合成的PbS@CdS@SiO2@F‐127纳米颗粒（NPs）具有水分散性，其量子产率为≈5.79%，远远大于大多数现有的NIR‐II荧光团。由于双层保护，PbS@CdS@SiO2@F‐127 NPs在广泛的pH值范围内表现出优异的化学稳定性。研究了PbS@CdS@SiO2@F‐127 NPs的生物相容性，结果表明NPs在体内的毒性可能很小。PbS@CdS@SiO2@F‐127 NPs随后被用于小鼠大脑的活体和实时NIR‐II荧光显微成像。血管结构可视化，成像深度达到950µm。此外，通过将相当低剂量的PbS@CdS@SiO2@F‐127 NPs灌注到小鼠体内，可以实现胃肠道的体内NIR‐II荧光成像。这项工作说明了超稳定、生物相容性和明亮的NIR - II量子点在生物医学和临床应用中的潜力，这些应用需要深层组织成像。
Fluorescence bioimaging in the second near‐infrared spectral region (NIR‐II, 1000–1700 nm) can provide advantages of high spatial resolution and large penetration depth, due to low light scattering. However, NIR‐II fluorophores simultaneously possessing high brightness, good stability, and biocompatibility are very rare. Hydrophobic NIR‐II emissive PbS@CdS quantum dots (QDs) are surface‐functionalized, via a silica and amphiphilic polymer (Pluronic F‐127) dual‐layer coating method. The as‐synthesized PbS@CdS@SiO2@F‐127 nanoparticles (NPs) are aqueously dispersible and possess a quantum yield of ≈5.79%, which is much larger than those of most existing NIR‐II fluorophores. Thanks to the dual‐layer protection, PbS@CdS@SiO2@F‐127 NPs show excellent chemical stability in a wide range of pH values. The biocompatibility of PbS@CdS@SiO2@F‐127 NPs is studied, and the results show that the toxicity of the NPs in vivo could be minimal. PbS@CdS@SiO2@F‐127 NPs are then utilized for in vivo and real‐time NIR‐II fluorescence microscopic imaging of mouse brain. The architecture of blood vessels is visualized and the imaging depth reaches 950 µm. Furthermore, in vivo NIR‐II fluorescence imaging of gastrointestinal tract is achieved, by perfusing PbS@CdS@SiO2@F‐127 NPs into mice at a rather low dosage. This work illustrates the potential of ultrastable, biocompatible, and bright NIR‐II QDs in biomedical and clinical applications, which require deep tissue imaging.