Multivalent nanoparticle networks enable point-of-care detection of human phospholipase-A2 in serum.
Multivalent nanoparticle networks enable point-of-care detection of human phospholipase-A2 in serum.
复制标题
DOI：
10.1021/nn5057595
复制
发表时间：
2015-03-24
期刊：
影响因子：
17.1
通讯作者：
Stevens MM
中科院分区：
文献类型：
--
作者：
Chapman R;Lin Y;Burnapp M;Bentham A;Hillier D;Zabron A;Khan S;Tyreman M;Stevens MM
A rapid and highly sensitive point of care (PoC) lateral flow assay for phospholipase-A2 (PLA2) is demonstrated in serum through the enzyme-triggered release of a new class of biotinylated multi-armed polymers from a liposome substrate. Signal from the enzyme activity is generated by the adhesion of polystreptavidin coated gold nanoparticle networks to the lateral flow device, which leads to the appearance of a red test line due to the localised surface plasmon resonance (LSPR) effect of the gold. The use of a liposome as the enzyme substrate and multivalent linkers to link the nanoparticles leads to amplification of the signal as the cleavage of a small amount of lipids is able to release a large amount of polymer linker and adhesion of an even larger amount of gold nanoparticles. By optimising the molecular weight and multivalency of these biotinylated polymer linkers the sensitivity of the device can be tuned to enable naked-eye detection of 1 nM human-PLA2 in serum within 10 minutes. This high sensitivity enabled the correct diagnosis of pancreatitis in diseased clinical samples against a set of healthy controls using PLA2 activity in a point of care device for the first time.
