Formation of occlusive platelet aggregates in whole blood caused by low concentrations of ADP.
Formation of occlusive platelet aggregates in whole blood caused by low concentrations of ADP.
复制标题
低浓度 ADP 导致全血中形成闭塞性血小板聚集体。
DOI：
10.1097/00002480-200011000-00008
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Mohammad,SF
中科院分区：
文献类型：
--
作者：
Hall,MW;Goodman,PD;Solen,KA;Mohammad,SF
当血小板在体外流动过程中受到剪切应力时，就会释放出微量浓度的ADP。然而，基于目前的方法，这些低浓度尚未显示对血小板功能有显著影响。我们在这里报告了刚性微聚集体（MA）的形成，以响应低浓度的ADP。采用新研制的光散射全血聚集仪（LSWBA）检测肝素化（1.5 u/ml）人血中ADP （0 ~ 2 μM）的聚集剂量响应。虽然LSWBA显示ADP诱导的MA是可逆的，但恒压过滤（50 mm Hg）提供的证据表明，聚体在血液中以刚性颗粒的形式存在长达6分钟。讨论了这些发现对体外循环的可能影响。
Minute concentrations of ADP are released when platelets are exposed to shear stress during extracorporeal flow. However, based on current methods, these low concentrations have not been shown to have a significant impact on platelet function. We report here the formation of rigid microaggregates (MA) in response to low concentrations of ADP. A newly developed light scattering whole blood aggregometer (LSWBA) was used to detect an aggregation dose response to ADP (0–2 μM) in heparinized (1.5 u/ml) human blood. Although the LSWBA showed that ADP induced MA were reversible, evidence provided by constant pressure filtration (50 mm Hg) suggested that aggregates existed as rigid particles in the blood for up to 6 minutes. The possible implications of these findings to extracorporeal circulation are discussed.