Molecular characterization of tree peony germplasm using sequence-related amplified polymorphism markers
Molecular characterization of tree peony germplasm using sequence-related amplified polymorphism markers
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DOI：
10.1007/s10528-007-9140-8
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发表时间：
2008-04-01
影响因子：
2.4
通讯作者：
Tetsumura, Takuya
中科院分区：
文献类型：
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作者：
Han, Xiao Yan;Wang, Liang Sheng;Tetsumura, Takuya
本研究利用序列相关扩增多态性（SRAP）分子标记对63份牡丹品种（包括3个野生种和63个栽培种）进行了基因组多态性检测。用23对SRAP引物对每个样本的批量DNA样品进行了评估。在296个不同的扩增子中，262个是多态性的。最大简约，邻居加入，和未加权对组的方法，算术平均树在很大程度上是一致的。在这3棵树中，野生种长叶山海棠和高山山海棠形成了独立的聚类，具有很强的自举支持，而高山山海棠与所有栽培种都有很近的亲缘关系。根据不同的自举值将品种划分为不同的类群。基因型间的遗传相似性在0.02~0.73之间。这些结果表明，SRAP标记可以有效地检测牡丹基因组多态性，为牡丹遗传连锁图谱的构建和分子标记辅助选择育种提供了依据。
This study examined 63 tree peony specimens, consisting of 3 wild species and 63 cultivars, using sequence-related amplified polymorphism (SRAP) markers for the purpose of detecting genomic polymorphisms. Bulk DNA samples from each specimen were evaluated with 23 SRAP primer pairs. Among the 296 different amplicons, 262 were polymorphic. The maximum parsimony, neighbor-joining, and unweighted pair-group method using arithmetic average trees were largely in congruence. In the three trees, the wild species Paeonia ludlowii and P. delavayi formed separate clusters with strong bootstrap support, and P. ostii was closely related to all cultivars. The cultivars were divided into groups with various corresponding bootstrap values. The genetic similarity among the genotypes ranged from 0.02 to 0.73. These results demonstrate that SRAP markers are effective in detecting genomic polymorphisms in the tree peony and should be useful for linkage map construction and molecular marker assisted selection breeding.