Expression of extracellular matrix metalloproteinase inducer and enhancement of the production of matrix metalloproteinase-1 in tongue squamous cell carcinoma
Expression of extracellular matrix metalloproteinase inducer and enhancement of the production of matrix metalloproteinase-1 in tongue squamous cell carcinoma
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DOI：
10.1016/j.ijom.2009.03.004
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发表时间：
2009-08-01
影响因子：
2.4
通讯作者：
Li, C.
中科院分区：
文献类型：
--
作者：
Cao, Z.;Xiang, J.;Li, C.
近年来研究发现，金属蛋白酶-1（MMP-1）的过度表达除了促进细胞侵袭外，还与肿瘤的早期发展有关。细胞外基质金属蛋白酶诱导因子（extracellular matrix metalloproteinase inducer，EMMPRIN）是一种跨膜糖蛋白，在肿瘤细胞中高度表达，可诱导肿瘤周围成纤维细胞（peritumor fibroblasts，PTFs）产生MMPs。本研究探讨了EMMPRIN在舌鳞癌中的表达。结果发现EMMPRIN在舌鳞癌的整个病变组织中均表达于细胞膜上。免疫荧光染色定位EMMPRIN的细胞膜在一个高度侵袭性的舌鳞状细胞癌细胞系（Tca 8113）。EMMPRIN mRNA在Tca 8113中高水平表达，而MMP-1 mRNA在Tca 8113的PTF中表达，但较难检测到。Tea 8113与PTF的共培养刺激MMP-1的产生。EMMPRIN在舌鳞癌中高表达，并可诱导局部MMP-1的产生。提示EMMPRIN可能在舌鳞癌的发生、发展和侵袭中起重要作用。
Recent Studies have found that ill addition to promoting Cellular invasion, overexpression of metalloproteinase-1 (MMP-1) is associated with the initial stages of cancer development. Extracellular matrix metalloproteinase inducer (EMMPRIN), a transmembrane glycoprotein, has been reported to be highly expressed in tumor cells and induce production of MMPs front peritumor fibroblasts (PTFs) adjacent to the tumor cells. The expression or EMMPRIN in tongue squamous Cell carcinoma (SCC) was investigated in this study. It was found that EMMPRIN was expressed at the cell membrane throughout the entire lesion ill tongue SCC. Immunofluorescence staining localized EMMPRIN to the cell membrane in a highly invasive tongue SCC cell line (Tca 8113). EMMPRIN mRNA was expressed at a high level in Tca 8113, whereas MMP-1 mRNA was expressed ill PTF but harder to be detected in Tca 8113. Co-culture of Tea 8113 with PTF stimulated production of MMP-1. EMMPRIN was highly expressed in tongue SCC, and could induce local production of MMP-1. These data indicate that EMMPRIN might play an important role in tongue SCC progression and invasion.