Characterization of wastewater toxicity by means of a whole-cell bacterial biosensor, using Pseudomonas putida, in conjunction with chemical analysis
Characterization of wastewater toxicity by means of a whole-cell bacterial biosensor, using Pseudomonas putida, in conjunction with chemical analysis
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DOI：
10.1007/s002160100925
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发表时间：
2001-10-01
期刊：
影响因子：
--
通讯作者：
Barceló, D
中科院分区：
文献类型：
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作者：
Farré, M;Barceló, D
基于对恶臭假单胞菌的抑制作用，研制了一种新型的电流型生物传感器，用于评价废水的急性毒性。该系统使用固定在一次性丝网印刷电极上的生物组分。以恶臭假单胞菌为生物组分，研究了对一组极性有机标准物质的响应。结果进行了比较，使用相同的系统和大肠杆菌作为生物成分，并与生物发光抑制弧菌的ToxAlert 100。计算并比较了不同性质，如数据的标准偏差（SD）、拟合优度（W）和抑制曲线各点垂直距离的标准偏差（Syx）、标准品的50%有效浓度（EC 50）和毒性单位（TII 50）。利用该生物传感器对不同污水处理厂收集的真实的废水样品进行了急性毒性评估，最后采用连续固相萃取（SSPE）和液相色谱-质谱（LC-MS）方法测定了废水样品中存在的极性有机有毒物质。
A new amperometric biosensor based on inhibition of Pseudomonas putida has been developed to assess the acute toxicity of wastewater. This system uses the biological component immobilized on disposable screen-printed electrodes. The responses for a selected group of polar organic standard substances were studied using Pseudomonas putida as biological component. The results have been compared with responses obtained using the same system and Escherichia coli as biological component and with the bioluminescence inhibition of Vibrio fisheri using ToxAlert 100. Different properties, e.g. the standard deviation (SD) of the data, the goodness of fit (W) and the standard deviation (Syx) of the vertical distances of the points from the inhibition curve, the 50% effective concentration (EC50) and the toxicity units (TII50) of the standard substance, were calculated and compared. This biosensor was used to assess the acute toxicity of real wastewater samples collected at different wastewater treatment plants (WWTP).Finally, a sequential solid-phase extraction (SSPE) procedure followed by liquid chromatography-mass spectrometry (LC-MS) was used to determine the polar organic toxic substances present in the wastewater samples.