Visualization and analysis of gene expression in tissue sections by spatial transcriptomics
Visualization and analysis of gene expression in tissue sections by spatial transcriptomics
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DOI：
10.1126/science.aaf2403
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发表时间：
2016-07-01
期刊：
影响因子：
56.9
通讯作者：
Frisen, Jonas
中科院分区：
文献类型：
--
作者：
Stahl, Patrik L.;Salmen, Fredrik;Frisen, Jonas
组织切片中蛋白质或信使RNA（mRNA）模式的分析是生物医学研究和诊断的基石。这通常涉及一次对一些蛋白质或表达基因的可视化。我们设计了一种策略，我们称之为“空间转录组学”，它允许在单个组织切片中以空间分辨率对转录组进行可视化和定量分析。通过将组织学切片定位在具有独特位置条形码的阵列逆转录引物上，我们展示了高质量的RNA测序数据，其中保持了来自小鼠脑和人类乳腺癌的二维位置信息。空间转录组学提供定量基因表达数据和组织切片内mRNA分布的可视化，并实现新型生物信息学分析，在研究和诊断中具有价值。
Analysis of the pattern of proteins or messenger RNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics. This typically involves the visualization of a few proteins or expressed genes at a time. We have devised a strategy, which we call "spatial transcriptomics," that allows visualization and quantitative analysis of the transcriptome with spatial resolution in individual tissue sections. By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, we demonstrate high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics.