Confining domains lead to reaction bursts: reaction kinetics in the plasma membrane.
Confining domains lead to reaction bursts: reaction kinetics in the plasma membrane.
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DOI：
10.1371/journal.pone.0032948
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kusumi A
中科院分区：
文献类型：
--
作者：
Kalay Z;Fujiwara TK;Kusumi A
将分子限制在细胞内特定的小体积和区域中可能是在进化过程中开发的用于调节生物分子的相互作用和功能的一般策略。细胞质膜是包围整个细胞的最外层膜，被认为是连续的二维液体，但越来越清楚的是，它由许多具有不同寿命的纳米-介观尺度域组成，例如筏域和细胞因子诱导的隔室，并且膜分子被动态地捕获在这些域中。在这篇文章中，我们给出了一个理论帐户的影响，分子限制可逆的双分子反应在一个分区表面，如质膜。通过基于晶格的扩散和反应模型进行模拟，我们发现，在存在膜分区的情况下，在每个隔室中发生的双分子反应以突发方式进行，在此期间，反应速率急剧而短暂地增加，即使渐近反应速率保持不变。我们的特点是反应爆发之间的时间和爆发的幅度作为模型参数的函数，并讨论了存在强抑制剂活性的反应爆发的生物学意义。
Confinement of molecules in specific small volumes and areas within a cell is likely to be a general strategy that is developed during evolution for regulating the interactions and functions of biomolecules. The cellular plasma membrane, which is the outermost membrane that surrounds the entire cell, was considered to be a continuous two-dimensional liquid, but it is becoming clear that it consists of numerous nano-meso-scale domains with various lifetimes, such as raft domains and cytoskeleton-induced compartments, and membrane molecules are dynamically trapped in these domains. In this article, we give a theoretical account on the effects of molecular confinement on reversible bimolecular reactions in a partitioned surface such as the plasma membrane. By performing simulations based on a lattice-based model of diffusion and reaction, we found that in the presence of membrane partitioning, bimolecular reactions that occur in each compartment proceed in bursts during which the reaction rate is sharply and briefly increased even though the asymptotic reaction rate remains the same. We characterized the time between reaction bursts and the burst amplitude as a function of the model parameters, and discussed the biological significance of the reaction bursts in the presence of strong inhibitor activity.
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