CONNEXIN40, A COMPONENT OF GAP-JUNCTIONS IN VASCULAR ENDOTHELIUM, IS RESTRICTED IN ITS ABILITY TO INTERACT WITH OTHER CONNEXINS
CONNEXIN40, A COMPONENT OF GAP-JUNCTIONS IN VASCULAR ENDOTHELIUM, IS RESTRICTED IN ITS ABILITY TO INTERACT WITH OTHER CONNEXINS
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DOI：
10.1091/mbc.4.1.7
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发表时间：
1993-01-01
影响因子：
3.3
通讯作者：
PAUL, DL
中科院分区：
文献类型：
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作者：
BRUZZONE, R;HAEFLIGER, JA;PAUL, DL
采用两种不同的特异性抗肽抗体，利用免疫荧光显微镜检测了新克隆的缝隙连接结构蛋白connexin40 （Cx40）的细胞分布。在肌肉和弹性动脉的内皮中检测到Cx40，其点状分布与已知的间隙连接分布一致。然而，在血管壁的其他细胞中未检测到。相比之下，另一种存在于心血管系统的连接蛋白Cx43在肌性动脉内皮细胞中未检测到，但在心肌和主动脉平滑肌中含量丰富。我们已经测试了这些连接的功能相互作用的能力。Cx40在爪蟾卵母细胞对中功能表达，并诱导形成具有独特电压依赖性的细胞间通道。出乎意料的是，当表达Cx40的卵母细胞与表达Cx43的卵母细胞配对时，虽然它们都可以与不同的连接蛋白Cx37相互作用，在配对的卵母细胞中形成间隙连接通道，但并没有发生通信。这些发现表明，细胞间通讯的建立可以通过不同连接蛋白的选择性表达在空间上进行调节，并提示了一种可能控制细胞间通讯程度的机制。
The cellular distribution of connexin40 (Cx40), a newly cloned gap junction structural protein, was examined by immunofluorescence microscopy using two different specific anti-peptide antibodies. Cx40 was detected in the endothelium of muscular as well as elastic arteries in a punctate pattern consistent with the known distribution of gap junctions. However, it was not detected in other cells of the vascular wall. By contrast, Cx43, another connexin present in the cardiovascular system, was not detected in endothelial cells of muscular arteries but was abundant in the myocardium and aortic smooth muscle. We have tested the ability of these connexins to interact functionally. Cx40 was functionally expressed in pairs of Xenopus oocytes and induced the formation of intercellular channels with unique voltage dependence. Unexpectedly, communication did not occur when oocytes expressing Cx40 were paired with those expressing Cx43, although each could interact with a different connexin, Cx37, to form gap junction channels in paired oocytes. These findings indicate that establishment of intercellular communication can be spatially regulated by the selective expression of different connexins and suggest a mechanism that may operate to control the extent of communication between cells.