Effect of bisphenol A on human endometrial stromal fibroblasts in vitro
Effect of bisphenol A on human endometrial stromal fibroblasts in vitro
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DOI：
10.1016/j.rbmo.2010.12.007
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发表时间：
2011-03-01
影响因子：
4
通讯作者：
Giudice, L. C.
中科院分区：
文献类型：
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作者：
Aghajanova, L.;Giudice, L. C.
本研究探讨双酚A（BPA）对人子宫内膜间质成纤维细胞（ESF）分化及雌激素代谢相关基因表达的影响。培养8例子宫切除标本的人ESF，并用5-100 μ mol/l BPA +/-雌二醇或8-br-cAMP处理48小时。通过实时逆转录PCR分析mRNA表达。8-br-cAMP诱导的人ESF蜕膜化通过胰岛素样生长因子结合蛋白-1（IGFBP 1）的表达和催乳素分泌来证实。短期暴露（48 h）可降低人ESF的增殖（P < 0.04），而不是由于凋亡。高剂量BPA显著诱导IGFBP 1 mRNA和蛋白表达，降低P450 SCC mRNA表达，以剂量依赖方式逆转8-br-cAMP诱导的HSD 17 B2（雌二醇向雌酮的转化）增加，并下调HSD 17 B1表达（雌酮向雌二醇的转化; P
This study evaluated the effects of bisphenol A (BPA) on human endometrial stromal fibroblast (ESF) differentiation and expression of genes involved in oestrogen metabolism. Human ESF from eight hysterectomy specimens were cultured and treated with 5-100 mu mol/l of BPA +/- oestradiol or 8-br-cAMP for 48 h. mRNA expression was analysed by real-time reverse-transcription PCR. 8-br-cAMP-induced human ESF decidualization was confirmed by expression of insulin-like growth factor binding protein-1 (IGFBP1) and prolactin secretion. Short-term exposure (48 h) decreased human ESF proliferation (P < 0.04) not due to apoptosis. High doses of BPA significantly induced IGFBP1 mRNA and protein, decreased P450scc mRNA, reversed the 8-br-cAMP-induced increase in HSD17B2 (oestradiol to oestrone conversion) in a dose-dependent manner and down-regulated HSD17B1 expression (oestrone to oestradiol conversion; P