Construction of Commercial Sweet Cherry Linkage Maps and QTL Analysis for Trunk Diameter.
Construction of Commercial Sweet Cherry Linkage Maps and QTL Analysis for Trunk Diameter.
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商品甜樱桃连锁图谱构建及树干直径QTL分析
DOI：
10.1371/journal.pone.0141261
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Duan X
中科院分区：
文献类型：
--
作者：
Wang J;Zhang K;Zhang X;Yan G;Zhou Y;Feng L;Ni Y;Duan X
对甜樱桃（Prunus avium）品种“Wanhongzhu”和“Lapins”进行杂交，以创建适合构建连锁图谱的作图群体。特定位点扩增片段 (SLAF) 测序技术用作单核苷酸多态性 (SNP) 发现平台，并生成了 701 个信息丰富的基因型检测；这些与 16 个微卫星 (SSR) 和不相容性 (S) 基因一起用于构建包含 8 个连锁群 (LG) 且遗传距离为 849.0 cM 的图谱。平均标记间距离为 1.18 cM，并且很少有长度 > 5 cM 的间隙。标记共线性与已建立的桃基因组序列保持一致。该图谱用于显示树干直径 (TD) 受 4 个基因座控制，映射到 3 个不同的 LG。不同的位点在树木发育的不同阶段影响TD。高密度“W×L”遗传连锁图谱有可能实现农艺相关性状QTL的高分辨率鉴定，并加速甜樱桃育种。
A cross between the sweet cherry (Prunus avium) cultivars ‘Wanhongzhu’ and ‘Lapins’ was performed to create a mapping population suitable for the construction of a linkage map. The specific-locus amplified fragment (SLAF) sequencing technique used as a single nucleotide polymorphism (SNP) discovery platform and generated 701 informative genotypic assays; these, along with 16 microsatellites (SSRs) and the incompatibility (S) gene, were used to build a map which comprised 8 linkage groups (LGs) and covered a genetic distance of 849.0 cM. The mean inter-marker distance was 1.18 cM and there were few gaps > 5 cM in length. Marker collinearity was maintained with the established peach genomic sequence. The map was used to show that trunk diameter (TD) is under the control of 4 loci, mapping to 3 different LGs. Different locus influenced TD at a varying stage of the tree’s development. The high density ‘W×L’ genetic linkage map has the potential to enable high-resolution identification of QTLs of agronomically relevant traits, and accelerate sweet cherry breeding.