GOLD(III) REACTIONS WITH NUCLEOTIDES AND POLYNUCLEOTIDES .1. GOLD(III) COMPLEXES OF ADENINE NUCLEOTIDES
GOLD(III) REACTIONS WITH NUCLEOTIDES AND POLYNUCLEOTIDES .1. GOLD(III) COMPLEXES OF ADENINE NUCLEOTIDES
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DOI：
10.1021/bi00796a006
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发表时间：
1971-01-01
期刊：
影响因子：
2.9
通讯作者：
BARRNETT, RJ
中科院分区：
文献类型：
--
作者：
GIBSON, DW;BEER, M;BARRNETT, RJ
Dean W. Gibson、f Michael Beer 和 Russell J. Barrnett 摘要：腺嘌呤、腺苷和腺嘌呤核苷酸（AMP、dAMP、3'AMP、cAMP、ADP 和 ATP）可在多个 pH+ pCl 下与羟基氯金酸离子的平衡系统反应。通过乙醇沉淀分离金-腺嘌呤核苷酸加合物的钾盐Au·A(K)，并分析金和磷酸盐的含量。在 pH 5-8 下含有 4 mM A 和 12 mM Au 离子的混合物中，4 小时后加合物含有 2 Au/A，24 小时后含有 3 Au/A。加合物似乎在某种程度上聚合，可能是由于 N Au «—N 桥。 Au·Ade 和 Au·Ado 加合物在水介质中非常难溶，而金-腺嘌呤核苷酸的钾盐可溶解至大于 10 mM [A]，Au-cAMP 的镁、钙和锰盐非常易溶于水，而 Au· AMP、Au· ADP 和 Au· ATP 的相应盐则相对难溶，其程度取决于磷酸盐和二价的数量。阳离子。 Au·A（K）加合物可以与氨基酸、相关胺以及含有伯环和杂环的生物染色剂进一步反应
Dean W. Gibson, f Michael Beer, and Russell J. Barrnett abstract: Adenine, adenosine, and adenine nucleotides (AMP, dAMP, 3'AMP, cAMP, ADP, and ATP) were allowed to react with equilibrium systems of hydroxochloroaurate ions, at several pH+ pCl. Potassium salts of gold-adenine nucleotide adducts, Au· A (K), were isolated by ethanol pre-cipitation, and analyzed for gold and phosphatecontents. In mixtures containing 4 mM A and 12 mM Au ions at pH 5-8, the adducts contained 2 Au/A after 4 hr, and 3 Au/A after 24 hr. The adducts appearto be polymerized to some extent, possibly because of N Au «—N bridges. The Au· Ade and Au· Ado adducts are very insoluble in aqueous media, whereas potassium salts of gold-adenine nucleotides are soluble to greater than 10 mM [A], Magnesium, calcium, and manganous salts of Au-cAMP are very water soluble, whereas the cor-responding salts of Au· AMP, Au· ADP, and Au· ATP are relatively insoluble, to degrees that depend on the number of phosphates and the divalent cation. The Au· A (K) adducts were allowed to react further with amino acids, related amines, and with biological stains containing primary and heterocyclic