Endophytic Actinomycetes from Azadirachta indica A. Juss.: Isolation, Diversity, and Anti-microbial Activity
Endophytic Actinomycetes from Azadirachta indica A. Juss.: Isolation, Diversity, and Anti-microbial Activity
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DOI：
10.1007/s00248-008-9450-3
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发表时间：
2009-05-01
影响因子：
3.6
通讯作者：
Gange, Alan C.
中科院分区：
文献类型：
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作者：
Verma, Vijay C.;Gond, Surendra K.;Gange, Alan C.
印楝内生放线菌的研究Juss.筛选并评估它们对一系列病原真菌和细菌的抗微生物活性。从20种植物中共获得55个分离物，其中60%对一种或多种病原真菌和细菌表现出抑制活性。放线菌最常从根中回收（占所有分离物的54.5%），其次是茎（23.6%）和叶（21.8%）。优势菌属为链霉菌属（49.09%），其次为链孢囊菌属（14.5%）、小双孢菌属（10.9%）、轮枝链霉菌属（5.5%）、囊单孢菌属（5.5%）和诺卡氏菌属（3.6%）。链霉菌分离物AzR 006、011和031（均来自根）对荧光假单胞菌具有急性活性，而AzR 027、032和051（也均来自根）对大肠杆菌具有活性。同时，从叶片中分离的诺卡氏菌AzL025对枯草芽孢杆菌具有拮抗作用。结果表明，在55个化合物中，有32个化合物具有广谱的抗菌活性，其中约4%的化合物对病原真菌和细菌有较强的抑制作用。链霉菌AzR 031、008和047、诺卡氏菌AzL 025和链孢囊菌AzR 021和048的分离物特别令人感兴趣，因为它们对根病原体（包括腐霉属和疫霉属）显示出显著的拮抗活性。本研究中的indica可用于开发针对一系列病原真菌和细菌的潜在生物控制剂，以及用于生产新型天然抗微生物化合物。这些结果不仅进一步加深了我们对植物-微生物相互作用的理解，而且还表明存在可用于生物技术、医药和农业工业的未开发的内生微生物资源。
Endophytic actinomycetes from Azadirachta indica A. Juss. were screened and evaluated for their anti-microbial activity against an array of pathogenic fungi and bacteria. A total of 55 separate isolates were obtained from 20 plants, and 60% of these showed inhibitory activity against one or more pathogenic fungi and bacteria. Actinomycetes were most commonly recovered from roots (54.5% of all isolates), followed by stems (23.6%), and leaves (21.8%). The dominant genus was Streptomyces (49.09% of all isolates), while Streptosporangium (14.5%), Microbispora (10.9%), Streptoverticillium (5.5%), Sacchromonospora sp. (5.5%), and Nocardia (3.6%) were also recovered. Streptomyces isolates AzR 006, 011, and 031 (all from roots) had acute activity against Pseudomonas fluorescens, while AzR027, 032, and 051 (also all from roots) showed activity against Escherichia coli. Meanwhile, an isolate of Nocardia sp. from leaves (AzL025) showed antagonism against Bacillus subtilis. Overall, 32 of the 55 were found to have broad spectrum significant antimicrobial activity, while about 4% of them showed strong and acute inhibition to pathogenic fungi and bacteria. Isolates of Streptomyces AzR031, 008, and 047, Nocardia sp. AzL025, and Streptosporangium sp. AzR 021 and 048 are of particular interest because they showed significant antagonistic activity against root pathogens, including Pythium and Phytophthora sp. Thus, many of the isolates recovered from A. indica in this study may be used in developing potential bio-control agents against a range of pathogenic fungi and bacteria and in the production of novel natural antimicrobial compounds. These results not only further our understanding of plant-microbe interactions but also indicate that there is an untapped resource of endophytic microorganisms that could be exploited in the biotechnological, medicinal, and agricultural industries.