Novel PVA-DNA nanoparticles prepared by ultra high pressure technology for gene delivery
Novel PVA-DNA nanoparticles prepared by ultra high pressure technology for gene delivery
复制标题
DOI：
10.1016/j.msec.2004.08.046
复制
发表时间：
2004-12-01
期刊：
影响因子：
--
通讯作者：
Kishida, A
中科院分区：
文献类型：
--
作者：
Kimura, T;Okuno, A;Kishida, A
采用超高压（UHP）技术制备了聚乙烯醇(PVA)-DNA纳米颗粒。采用不同分子量（Mw）和不同皂化度（DS）的DNA和PVA混合溶液，在40℃、10000个大气压（981 MPa）条件下处理10 min。琼脂糖凝胶电泳和扫描电镜观察表明，PVA-DNA形成了平均直径约200微米的纳米颗粒。使用更高Mw和皂化度的PVA，纳米颗粒的形成量增加。纳米粒子形成的驱动力是DNA与PVA之间的氢键作用。为了将PVA-DNA纳米颗粒用于基因传递，采用Raw264细胞株研究了PVA-DNA纳米颗粒的细胞毒性和细胞摄取。PVA-DNA纳米颗粒的存在不影响细胞活力。荧光显微镜观察纳米颗粒内化到细胞内的情况。这些结果表明，UHP技术制备的PVA-DNA纳米颗粒可作为药物载体，特别是基因载体。(C) 2004 Elsevier B.V.版权所有
Polyvinyl alcohol (PVA)-DNA nanoparticles have been developed by ultra high pressure (UHP) technology. Mixture solutions of DNA and PVA having various molecular weights (Mw) and degree of saponifications (DS) were treated under 10,000 atmospheres (981 MPa) condition at 40 degreesC for 10 min. Agarose gel electrophoresis and scanning electron microscope observation revealed that the PVA-DNA nanoparticles with average diameter of about 200 run were formed. Using PVA of higher Mw and degree of saponifications, the amount of nanoparticles formed increased. The driving force of nanoparticle formation was the hydrogen bonding between DNA and PVA. In order to apply the PVA-DNA nanoparticles for gene delivery, the cytotoxicity and the cellular uptake of them were investigated using Raw264 cell lines. The cell viability was not influenced whether the presence of the PVA-DNA nanoparticles. Further, the nanoparticles internalized into cells were observed by fluorescent microscope. These results indicates that the PVA-DNA nanoparticles prepared by UHP technology showed be useful as drug carrier, especially for gene delivery. (C) 2004 Elsevier B.V. All rights reserved.