Regulation of Escherichia coli secA by cellular protein secretion proficiency requires an intact gene X signal sequence and an active translocon
Regulation of Escherichia coli secA by cellular protein secretion proficiency requires an intact gene X signal sequence and an active translocon
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DOI：
10.1128/jb.180.19.5240-5242.1998
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发表时间：
1998-10-01
影响因子：
3.2
通讯作者：
Sarker, S
中科院分区：
文献类型：
--
作者：
Oliver, D;Norman, J;Sarker, S
SECA受大肠杆菌细胞蛋白质分泌熟练状态的翻译调控。这种调节是通过使SecA上游基因X的信号序列突变来探索的，X基因促进了SecA的翻译偶联。基因X信号序列突变是SecA表达的组成部分，而prlA等位基因部分恢复了SecA的调控。这些结果表明，前基因X蛋白与转位蛋白的相互作用是适当的SecA调控所必需的。此外，X基因信号序列突变仅在顺式构型中扰乱了SecA的调控。我们认为，新生的前基因X蛋白在其分泌过程中与转位蛋白相互作用，构成分泌感受器。
secA is translationally regulated by the protein secretion proficiency state of the Escherichia coli cell. This regulation was explored by making signal sequence mutations in the gene upstream of secA, gene X, which promotes secA translational coupling. Gene X signal sequence mutants were constitutive for secA expression, while prlA alleles partially restored secA regulation. These results show that interaction of the pre-gene X protein with the translocon is required for proper secA regulation. Furthermore, gene X signal sequence mutations disrupted secA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor.