STRUCTURE AND EVOLUTION OF 4 POU DOMAIN GENES EXPRESSED IN MOUSE-BRAIN
STRUCTURE AND EVOLUTION OF 4 POU DOMAIN GENES EXPRESSED IN MOUSE-BRAIN
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DOI：
10.1073/pnas.89.8.3280
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发表时间：
1992-04-15
影响因子：
11.1
通讯作者：
NIRENBERG, M
中科院分区：
文献类型：
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作者：
HARA, Y;ROVESCALLI, AC;NIRENBERG, M
克隆了在成年大脑中表达的四个小鼠POU结构域基因组DNA克隆——Brain-1、Brain-2、Brain-4和Scip-和Brain-2 cDNA，并对基因的编码区和非编码区进行了测序。 4个POU结构域的氨基酸序列高度保守；蛋白质其他区域的序列也保守，但程度较小。四个 POU 结构域基因的编码区缺乏内含子，并且相应蛋白质的氨基酸序列相似，表明祖先 III 类 POU 结构域基因的编码区缺乏内含子，因此可能起源于 POU 结构域 mRNA 分子的逆转录，然后将 cDNA 插入生殖细胞基因组 DNA。祖先 III 类 POU 结构域基因（或 mRNA）的额外重复将产生 Brain-1、Brain-2、Brain-4 和 Scip 基因。
Four mouse POU domain genomic DNA clones-Brain-1, Brain-2, Brain-4, and Scip-and Brain-2 CDNA, which are expressed in adult brain, were cloned and the coding and noncoding regions of the genes were sequenced. The amino acid sequences of the four POU domains are highly conserved; sequences in other regions of the proteins also are conserved but to a lesser extent. The absence Of introns from the coding regions of the four POU domain genes and the similarity of amino acid sequences of the corresponding proteins suggest that the coding region of the ancestral class III POU domain gene lacked introns and therefore may have originated by reverse transcription of a molecule of POU domain mRNA followed by insertion of the cDNA into germ cell genomic DNA. Additional duplications of the ancestral class III POU domain gene (or mRNA) would create the Brain-1, Brain-2, Brain-4, and Scip genes.