CYTOTOXIC EFFECTS OF HYPERTHERMIA AND ADRIAMYCIN ON CHINESE-HAMSTER CELLS
CYTOTOXIC EFFECTS OF HYPERTHERMIA AND ADRIAMYCIN ON CHINESE-HAMSTER CELLS
复制标题
DOI：
10.1093/jnci/57.5.1063
复制
发表时间：
1976-01-01
影响因子：
10.3
通讯作者：
STRANDE, DP
中科院分区：
文献类型：
--
作者：
HAHN, GM;STRANDE, DP
中国仓鼠细胞，暴露于同时高温（43 ℃）。C）和阿霉素（ADM），最初敏化ADM的细胞毒活性。如果联合治疗的持续时间超过约30分钟，细胞变得难治性额外的杀伤ADM。如果加热应用前ADM曝光，细胞也可以呈现不敏感。这种状态持续到在43 ℃下暴露50分钟后24小时。最合理的解释是，细胞膜对ADM的渗透性最初由高温增加，但延长（> 30分钟）热暴露逆转了这种情况，并抑制了额外的ADM渗透到敏感部位。这些数据没有提供任何关于所涉及的精确机制的线索，也没有足够的精确性来排除其他解释。结果表明，如果ADM和热疗的组合是在临床上用于癌症治疗，必须观察到的预防措施。
Chinese hamster cells, exposed to simultaneous hyperthermia (43.degree. C) and adriamycin (ADM), were initially sensitized to the cytotoxic activity of ADM. If the duration of the combined treatment exceeded about 30 min, the cells became refractory to additional killing by ADM. If heat was applied before ADM exposure, the cells also could be rendered insensitive. This state persisted so that 24 h after a 50 min exposure to 43.degree. C, the cells still showed considerable resistance to ADM. The most reasonable explanation for the findings was that cell membrane permeability to ADM was initially increased by hyperthermia, but prolonged (> 30 min) heat exposure reversed this situation and inhibited additional ADM from penetrating to sensitive sites. The data yield no hints as to the precise mechanisms involved nor are they sufficiently precise to exclude other explanations. The results suggest precautions that must be observed if the combination of ADM and hyperthermia is to be used clinically in cancer treatment.