The individual regulation of granule protein mRNA levels during neutrophil maturation explains the heterogeneity of neutrophil granules
The individual regulation of granule protein mRNA levels during neutrophil maturation explains the heterogeneity of neutrophil granules
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DOI：
10.1002/jlb.66.6.989
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发表时间：
1999-12-01
影响因子：
5.5
通讯作者：
Borregaard, N
中科院分区：
文献类型：
--
作者：
Cowland, JB;Borregaard, N
确定中性粒细胞分化过程中16个颗粒蛋白的体内mRNA水平。解决颗粒蛋白的合成是单独或阻滞的问题。从外周血粒细胞中提取RNA，并通过percoll密度离心从人体骨髓中分离出的三种不同的中性粒细胞前体，通过北斑（12个基质蛋白质骨髓氧化酶）确定与细胞成熟的mRNA水平相关的mRNA水平。蛋白酶3，弹性酶防御素，乳铁蛋白，NGAL，HCAP-18，Transcobalamin-I，SGP28，SGP28，明胶酶，溶菌酶和Serglycin和4膜蛋白蛋白CD68，CD11B，CD11B，N-甲酰基 - 甲基甲基 - 甲基甲基 - 甲基甲基苯甲酰丙糖苷基甲酰亚胺基甲酰胺基甲酯。和CD35，该小组的转录本可确保在研究中包括所有嗜中性粒细胞的所有外胞囊细胞器的标记。证明了颗粒蛋白的mRNA高度分化的分布，可以解释中性粒细胞的细胞内储存颗粒和分泌囊泡的异质性，此外，这些转录物的个别分布为中性粒细胞的详细评估提供了基础通过形态学研究或将单个标记蛋白用于葡萄糖，特异性和明胶酶颗粒。
The in vivo mRNA levels for 16 granule proteins during neutrophil differentiation were determined. to address the question of whether the synthesis of granule proteins is regulated individually or blockwise. RNA was extracted from peripheral blood granulocytes and three different populations of neutrophil precursors isolated from human bone marrow by Percoll density centrifugation, The mRNA levels in relation to the maturation of the cells were determined by Northern blot for the 12 matrix proteins myeloperoxidase. proteinase-3, elastase defensin, lactoferrin, NGAL, hCAP-18, transcobalamin-I, SGP28, gelatinase, lysozyme, and serglycin and the 4 membrane proteins CD68, CD11b, N-formyl-methionyl-leucyl-phenylalanine receptor. and CD35, This panel of transcripts ensured that markers for all exocytosable organelles of the neutrophil were included in the study. A highly differentiated distribution of mRNAs for granule proteins was demonstrated that can explain the heterogeneity of the intracellular storage granules and secretory vesicles of the neutrophil, Furthermore, the individual distribution of these transcripts provides the basis for a more detailed assessment of neutrophil maturation than that obtained by morphological studies or the use of a single marker protein for azurophil, specific, and gelatinase granules.