Polyphenols and triterpenes from Chaenomeles fruits: Chemical analysis and antioxidant activities assessment
Polyphenols and triterpenes from Chaenomeles fruits: Chemical analysis and antioxidant activities assessment
复制标题
木瓜果实中的多酚和三萜：化学分析和抗氧化活性评估
DOI：
10.1016/j.foodchem.2013.06.109
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发表时间：
2013-12-15
期刊：
影响因子：
8.8
通讯作者：
Wang, Liang-Sheng
中科院分区：
文献类型：
--
作者：
Du, Hui;Wu, Jie;Wang, Liang-Sheng
木瓜是木瓜属的水果，是一种珍贵的保健食品和中药。采用HPLC-DAD/ESI-MS/MS技术对5种野生木瓜果实的多酚类化合物进行了研究，共分离出24种多酚类化合物，其中20种为黄烷-3-醇类化合物（包括儿茶素、表儿茶素和原花青素低聚物）。通过两种酸催化裂解反应对原花青素的平均聚合度进行了测定，结果表明，木瓜的平均聚合度最高，而木瓜的最低。皱皮木瓜和西藏木瓜的总多酚含量分别为46.92和46.28 mg/g没食子酸当量（GAE），但它们的主要活性成分不同（皱皮木瓜的主要活性成分为表儿茶素和原花青素B2，西藏木瓜的主要活性成分为儿茶素和原花青素B1）。这两个物种也表现出同样强的自由基清除活性。木瓜果实的抗氧化能力与其总多酚含量呈显著正相关。两种三萜类化合物（油酸和熊果酸）分别在木瓜和木瓜中含量最高。(C)2013爱思唯尔有限公司版权所有。
Mugua, fruit of the genus Chaenomeles, is a valuable source of health food and Chinese medicine. To elucidate the bioactive compounds of five wild Chaenomeles species, extracts from fresh fruits were investigated by HPLC-DAD/ESI-MS/MS. Among the 24 polyphenol compounds obtained, 20 were flavan-3-ols (including catechin, epicatechin and procyanidin oligomers). The mean polymerisation degree (mDP) of procyanidins was examined by two acid-catalysed cleavage reactions; the mDP value was the highest in Chaenomeles sinensis and the lowest in Chaenomeles japonica. Total polyphenol content (TPC) reached 46.92 and 46.28 mg/g gallic acid equivalents (GAE) in Chaenomeles speciosa and Chaenomeles thibetica, respectively, although their main bioactive compounds were different (being epicatechin and procyanidin B2 in the former, and catechin and procyanidin B1 in the latter). These two species also exhibited equally strong free radical scavenging activities. Our results further showed that the antioxidant ability of Chaenomeles fruits was significantly correlated to their total polyphenol contents. Two triterpenes (oleanolic acid and ursolic acid) were the highest quantity in Chaenomeles cathayensis and C thibetica, respectively. (C) 2013 Elsevier Ltd. All rights reserved.