Synthesis and biological evaluation of the Zn (II)-IDB complexes appended with oligopolyamide as potent artificial nuclease.
Synthesis and biological evaluation of the Zn (II)-IDB complexes appended with oligopolyamide as potent artificial nuclease.
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DOI：
10.1016/j.bmcl.2008.09.074
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发表时间：
2008-11
影响因子：
2.7
通讯作者：
C. Li;R. Qiao;Ya-qin Wang;Yufen Zhao;R. Zeng
中科院分区：
文献类型：
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作者：
C. Li;R. Qiao;Ya-qin Wang;Yufen Zhao;R. Zeng
锌 (II) 复合物包含通过柔性接头缀合的寡聚酰胺和双(2-苯并咪唑基甲基)胺 (IDB)，已成功合成、表征和评估其作为 DNA 切割剂的作用。通过电泳研究了这些复合物对 DNA 的切割活性。结果表明，与没有低聚酰胺的情况相比，锌(II)配合物的裂解活性增强。特别是，在高反应浓度（1.2mM）下，Zn（II）复合物可以具有一定选择性地切割质粒DNA。此外，光谱数据表明，具有寡聚酰胺主链的 Zn (II) 复合物具有富含 A-T（腺嘌呤和胸腺嘧啶）的序列偏好。
The zinc (II) complexes, which contain oligopolyamide and bis(2-benzimidazolylmethyl)amine (IDB) conjugated by flexible linker, have been successfully synthesized, characterized, and evaluated as DNA cleavage agents. The cleavage activity of these complexes on DNA was studied by electrophoresis. The results showed that the cleavage activity of zinc (II) complexes was enhanced comparing with those without oligopolyamide. Specially, at a high reaction concentration (1.2mM), Zn (II) complex can cleave the plasmid DNA bearing some selectivity. Further, the spectroscopic data suggested that Zn (II) complexes with oligopolyamide backbone possessed A-T (adenine and thymine) rich sequences preference.