The Bradford Method For Protein Quantitation
The Bradford Method For Protein Quantitation
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DOI：
10.1007/978-1-59745-198-7_4
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发表时间：
2009-01-01
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影响因子：
--
通讯作者：
Kruger, Nicholas J.
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文献类型：
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作者：
Kruger, Nicholas J.
在蛋白质研究的许多领域中，快速准确地估计蛋白质浓度的方法是必不可少的。最初由Bradford（1）描述的测定法已成为许多实验室定量蛋白质的首选方法。该技术比Lowry方法更简单，更快速，更灵敏。此外，与Lowry法相比，它受普通试剂和生物样品非蛋白质组分的干扰较小（见注1）。
A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (seeNote 1).