A single linkage group confers dominant resistance to Bacillus thuringiensis δ‐endotoxin Cry1Ac in Helicoverpa armigera
A single linkage group confers dominant resistance to Bacillus thuringiensis δ‐endotoxin Cry1Ac in Helicoverpa armigera
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DOI：
10.1111/j.1439-0418.2008.01368.x
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发表时间：
2009-06
影响因子：
1.9
通讯作者：
Y. Wu;J. Vassal;M. Royer;I. Pieretti
中科院分区：
文献类型：
--
作者：
Y. Wu;J. Vassal;M. Royer;I. Pieretti
棉铃虫BKBT菌株来源于1977年产自科特迪瓦布瓦克的BK77敏感菌株，经活化的苏云金芽孢杆菌δ‐内毒素Cry1Ac筛选30代。与以往报道的棉铃虫Cry1Ac抗性的隐性遗传不同，BKBT菌株对活化Cry1Ac的抗性是显性的。采用回交法测定了BKBT菌株对Cry1Ac的显性抗性。147个信息丰富的扩增片段长度多态性（AFLP） DNA标记覆盖了棉铃虫所有31个连锁群。与BKBT株Cry1Ac抗性相关的5个AFLP标记在同一个常染色体连锁组上，这是棉铃虫BKBT株中唯一具有Cry1Ac抗性优势的连锁组。
The BKBT strain of Helicoverpa armigera was derived from a susceptible BK77 strain (collected from Bouake, Cote D’Ivoire in 1977) through 30 generations of selection with activated Bacillus thuringiensis δ‐endotoxin Cry1Ac. Unlike recessive inheritance of Cry1Ac resistance in H. armigera from previous reports, resistance to activated Cry1Ac in the BKBT strain is dominant. A backcross approach was used to map dominant resistance to Cry1Ac in the BKBT strain. One hundred and forty‐seven informative amplified fragment length polymorphism (AFLP) DNA markers covered all 31 linkage groups of H. armigera. Five AFLP markers linked to Cry1Ac resistance in the BKBT strain were on the same autosomal linkage group, which is the only linkage group contributing dominant Cry1Ac resistance in the BKBT strain of H. armigera.