Antibacterial and antiparasitic effects of Bothrops marajoensis venom and its fractions: Phospholipase A2 and L-amino acid oxidase
Antibacterial and antiparasitic effects of Bothrops marajoensis venom and its fractions: Phospholipase A2 and L-amino acid oxidase
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DOI：
10.1016/j.toxicon.2009.11.013
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发表时间：
2010-04-01
期刊：
影响因子：
2.8
通讯作者：
Martins, Alice M. C.
中科院分区：
文献类型：
--
作者：
Costa Torres, Alba Fabiola;Dantas, Rodrigo Tavares;Martins, Alice M. C.
蛇毒中存在的一些蛋白质具有酶活性，如磷脂酶A(2)和L-氨基酸氧化酶。在这项研究中，我们验证了马拉霍伯斯蛇毒(BmarTV)、解放军(2)(Bmarpla(2))和LAAO(BmarLAao)对细菌、酵母菌和利什曼原虫的作用。用Protein Pack 5PW对BmarTV进行分离，得到几个组分。反相高效液相色谱分析表明，从蛇毒中分离得到BmarPLA2(2)，其N端氨基酸序列与从蛇毒中分离到的其他赖氨酸K49SPLA2(2)S具有较高的氨基酸同源性。BmarLAao经纯化后分子均一，其N端氨基酸序列与其他LAAO具有高度的氨基酸保守性。BmarLAao对铜绿假单胞菌、白色念珠菌和金黄色葡萄球菌的生长有抑制作用，且呈剂量依赖关系。对金黄色葡萄球菌的抑制作用较强，最低抑菌浓度为50 mU g/mL，最低抑菌浓度为200 mU g/mL，而BmarTV和BmarPLA(2)对金黄色葡萄球菌无抑制作用。巴玛拉奥对L和L的前鞭毛体生长有抑制作用，其IC50值分别为2.55mU/m L和2.86mU/m L。BmarTV对日本血吸虫的IC50值为86.56mU/m L，对L的IC50为79.02m g/m L。Bmarpla(2)对这些寄生虫的生长没有任何抑制作用。BmarLaao和BmarTV在所研究的浓度下表现出低毒。综上所述，整个蛇毒及其L氨基酸氧化酶均能抑制金黄色葡萄球菌、白色念珠菌、铜绿假单胞菌和利什曼原虫等多种微生物的生长。(C)2009爱思唯尔有限公司。保留所有权利。
Some proteins present in snake venom possess enzymatic activities, such as phospholipase A(2) and L-amino acid oxidase. In this study, we verify the action of the Bothrops marajoensis venom (BmarTV), PLA(2) (BmarPLA(2)) and LAAO (BmarLAAO) on strains of bacteria, yeast, and Leishmania sp. The BmarTV was isolated by Protein Pack 5PW, and several fractions were obtained. Reverse phase HPLC showed that BmarPLA(2) was isolated from the venom, and N-terminal amino acid sequencing of sPLA(2) showed high amino acid identity with other lysine K49 sPLA(2)s isolated from Bothrops snakes. The BmarLAAO was purified to high molecular homogeneity and its N-terminal amino acid sequence demonstrated a high degree of amino acid conservation with others LAAOs. BmarLAAO was able to inhibit the growth of P. aeruginosa, C. albicans and S. aureus in a dose-dependent manner. The inhibitory effect was more significant on S. aureus, with a MIC = 50 mu g/mL and MLC = 200 mu g/mL However, the BmarTV and BmarPLA(2) did not demonstrate inhibitory capacity. BmarLAAO was able to inhibit the growth of promastigote forms of L chagasi and L amazonensis, with an IC50 = 2.55 mu g/mL and 2.86 mu g/mL for L. amazonensis and L. chagasi, respectively. BmarTV also provided significant inhibition of parasitic growth, with an IC50 of 86.56 mu g/mL for L. amazonensis and 79.02 mu g/mL for L chagasi. BmarPLA(2) did not promote any inhibition of the growth of these parasites. The BmarLAAO and BmarTV presented low toxicity at the concentrations studied. In conclusion, whole venom as well as the L-amino acid oxidase from Bothrops marajoensis was able to inhibit the growth of several microorganisms, including S. aureus, Candida albicans, Pseudomonas aeruginosa, and Leishmania sp. (C) 2009 Elsevier Ltd. All rights reserved.