Aligned 3D porous polyurethane scaffolds for biological anisotropic tissue regeneration
Aligned 3D porous polyurethane scaffolds for biological anisotropic tissue regeneration
复制标题
用于生物各向异性组织再生的对齐 3D 多孔聚氨酯支架
DOI：
10.1093/rb/rbz031
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发表时间：
2020-02-01
影响因子：
6.7
通讯作者：
Fu, Qiang
中科院分区：
文献类型：
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作者：
Lin, Weiwei;Lan, Wanling;Fu, Qiang
在组织工程领域，需要一种绿色的人工支架制造工艺(有机无溶剂)。本工作以可生物降解水性聚氨酯(PU)乳液为原料，采用定向冷冻干燥法制备了一系列定向三维支架，以确保不产生有机副产物。在优化了聚合物在乳液中的浓度并考察了不同的冻结温度后，根据所需的定向多孔结构(孔径为32.5µm/-9.3µm，孔隙率为92%)以及水平方向(强度为41.3kPA，模数为72.3kPA)和垂直方向(强度为45.5kPA，模数为139.3 kPA)的平衡力学性能，选择了聚合物含量为14wt%，在-196℃处理的定向PUS支架(PU14)。L929细胞的反应和肌肉组织的再生表明，这种基于纯材料的定向三维支架在体外和体内都可以促进定向细胞的发育和各向异性组织的再生。因此，这些具有有序结构的纯材料支架在肌肉、神经、脊髓等生物各向异性组织再生的组织工程中具有巨大的潜力。
A green fabrication process (organic solvent-free) of artificial scaffolds is required in tissue engineering field. In this work, a series of aligned three-dimensional (3D) scaffolds are made from biodegradable waterborne polyurethane (PU) emulsion via directional freeze-drying method to ensure no organic byproducts. After optimizing the concentration of polymer in the emulsion and investigating different freezing temperatures, an aligned PUs scaffold (PU14) generated from 14wt% polymer content and processed at -196 degrees C was selected based on the desired oriented porous structure (pore size of 32.5 +/- 9.3 mu m, porosity of 92%) and balanced mechanical properties both in the horizontal direction (strength of 41.3 kPa, modulus of 72.3 kPa) and in the vertical direction (strength of 45.5 kPa, modulus of 139.3 kPa). The response of L929 cells and the regeneration of muscle tissue demonstrated that such pure material-based aligned 3D scaffold can facilitate the development of orientated cells and anisotropic tissue regeneration both in vitro and in vivo. Thus, these pure material-based scaffolds with ordered architecture have great potentials in tissue engineering for biological anisotropic tissue regeneration, such as muscle, nerve, spinal cord and so on.