Cytokeratin 17 mRNA expression has potential for diagnostic marker of oral squamous cell carcinoma
Cytokeratin 17 mRNA expression has potential for diagnostic marker of oral squamous cell carcinoma
复制标题
细胞角蛋白 17 mRNA 表达具有作为口腔鳞状细胞癌诊断标志物的潜力
DOI：
10.1007/s00432-007-0350-6
复制
发表时间：
2007
影响因子：
3.6
通讯作者：
J. Ries
中科院分区：
文献类型：
--
作者：
T. Toyoshima;E. Vairaktaris;E. Nkenke;K. Schlegel;F. Neukam;J. Ries
目的口腔鳞状细胞癌（OSCC）标志物的检测对OSCC的早期诊断和个体化治疗具有重要意义。细胞角蛋白（CK）如CK 19和CK 20是已知的有用的诊断和预后标志物的实体瘤。本研究的目的是评估进一步的CKs诊断OSCC的相关性。材料在10例OSCC和5例正常粘膜标本中，通过cDNA微阵列检测31个CK基因的表达模式，以确定最明显的过度表达CKs。除46例OSCC样本外，还使用SYBR绿色实时逆转录酶聚合酶链反应（RT qPCR）通过相对定量（RQ）对CK 17、CK 19和CK 20的结果进行了验证。CK表达与肿瘤分级的相关性carried.ResultscDNA微阵列分析显示，在所有的CKs，CK 17是上调最强的OSCC相比，正常样本，过度表达与诊断最显着相关（P= 0.002）。CK19和CK20在口腔鳞癌和正常口腔黏膜中的表达率无显著性差异。在56份实时定量PCR分析的样本中，CK 17在53份（94.6%）中过表达，CK 19在18份（32.1%）中过表达，CK 20在7份（12.5%）中过表达。CK17的过度表达与颈淋巴结转移有关（P< 0.05）。CK 19在T3、T4期、Ⅲ、Ⅳ期及低分化鳞癌中均呈高表达（P <0.0 5）。CK20的过度表达与颈淋巴结转移有关（P< 0.03）。RQ测定结果显示，CK17过表达的平均值显著高于其他CK（P< 0.01），且与T1、T2期口腔鳞癌显著相关（P< 0.03），Ⅰ、Ⅱ期患者结论CK 19可能与口腔鳞癌的临床进展及分化程度有关，CK 20与口腔鳞癌颈淋巴结转移有关。由于CK 17在口腔鳞癌组织中的表达明显上调，且过表达程度较强，因此CK 17可能是CK家族中最适合诊断口腔鳞癌的标志物。
PurposeDetermination of marker for identification of oral squamous cell carcinoma (OSCC) is important for early diagnosis and individual therapy. Cytokeratins (CKs) like CK 19 and CK 20 are known to be useful diagnostic and prognostic markers for solid tumors. The aim of this study was to evaluate the relevance of further CKs for diagnosis of OSCC.MaterialsIn 10 OSCC and 5 normal mucosal samples, the expression patterns of 31 CK genes were examined by cDNA microarray in order to identify CKs with most pronounced over-expression. The results were verified for CK 17, CK 19, and CK 20 in addition to 46 OSCC samples by relative quantification (RQ) using SYBR green real-time reverse transcriptase polymerase chain reaction (RT qPCR). A correlation of the CK expressions with the tumor classification was carried out.ResultscDNA microarray analyses showed that out of all CKs, CK 17 was up-regulated strongest in OSCC compared to normal samples, and over-expression was most significantly associated with diagnosis (P= 0.002). Expression rates of CK 19 and CK 20 were not significantly different between OSCC samples and normal samples. In 56 samples analyzed by real-time RT qPCR, CK 17 was over-expressed in 53 (94.6%), CK 19 in 18 (32.1%), and CK 20 in 7 (12.5%). The over-expression of CK 17 was significantly associated with metastases of neck lymph nodes (P< 0.05). CK 19 was significantly over-expressed in T3 and T4 OSCC, in stage III and IV patients (P< 0.05), and in poorly differentiated OSCC (P< 0.03). The over-expression of CK 20 was significantly associated with metastases of neck lymph nodes (P< 0.03). Determined by RQ, the mean value of CK 17 over-expression was significantly higher than that of the other CKs (P< 0.01), and was significantly associated with T1 and T2 OSCC (P< 0.03) and with stage I and II patients (P< 0.01).ConclusionCK 19 might be linked to the clinical progression and differentiation of OSCC, and CK 20 could be associated with metastases of neck lymph nodes in OSCC. Due to the significant up-regulation and the strong over-expression, CK 17 might be the most suitable marker for diagnosis of OSCC out of the CK-family.