The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis
The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis
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DOI：
10.1046/j.1365-2958.2001.02709.x
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发表时间：
2001-12-01
影响因子：
3.6
通讯作者：
Lazazzera, BA
中科院分区：
文献类型：
--
作者：
Hamon, MA;Lazazzera, BA
生物膜是包裹在聚合物基质中并粘附于表面、界面或彼此的结构化细胞群落。我们在这里报告，土壤细菌枯草芽孢杆菌形成生物膜。通过激光共聚焦扫描显微镜观察到B.枯草芽孢杆菌粘附于非生物表面并形成深度大于或等于30 μ m的三维结构。这些生物膜似乎至少部分包裹在细胞外多糖基质中，因为它们可以用Calcofluor（一种多糖结合染料）染色。为了了解生物膜形成的分子机制，我们筛选了先前表征的生物膜形成缺陷的突变体。我们发现，spo0A，编码主要的早期孢子形成转录因子的突变，导致生物膜形成的缺陷。spo0A突变细胞以单层细胞而不是三维生物膜粘附于表面。Spo0A对生物膜发育的需求似乎是由于其在负调节AbrB中的作用。abrB的突变抑制了spo0A突变体的生物膜缺陷，这表明AbrB负调控至少一个基因，该基因是从单层附着细胞向成熟生物膜过渡所需的。生物膜发育对B生态学的影响。subtilis的讨论。
Biofilms are structured communities of cells encased in a polymeric matrix and adherent to a surface, interface or each other. We report here that the soil bacterium Bacillus subtilis forms biofilms. By confocal scanning laser microscopy, we observed that B. subtilis adhered to abiotic surfaces and formed a three-dimensional structure greater than or equal to 30 mum in depth. These biofilms appeared to be at least partly encased in an extracellular polysaccharide matrix, as they could be stained with Calcofluor, a polysaccharide-binding dye. To understand the molecular mechanism of biofilm formation, we screened previously characterized mutants for a defect in biofilm formation. We found that mutations in spo0A, which encodes the major early sporulation transcription factor, caused a defect in biofilm formation. spo0A mutant cells adhered to a surface in a monolayer of cells rather than a three-dimensional biofilm. The requirement of Spo0A for biofilm development appears to result from its role in negatively regulating AbrB. Mutations in abrB suppressed the biofilm defect of a spo0A mutant, indicating that AbrB negatively regulates at least one gene that is required for the transition from a monolayer of attached cells to a mature biofilm. Implications of biofilm development for the ecology of B. subtilis are discussed.